Functional analysis of the gene related with the flavonoid metabolism in grape berries

2014 Fiscal Year Final Research Report

• Project/Area Number: 23780036
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Horticulture/Landscape architecture
• Research Institution: National Research Institute of Brewing
• Principal Investigator: KAZUYA Koyama 独立行政法人酒類総合研究所, その他部局等, 主任研究員 (30416424)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 二次代謝 / ブドウ / ストレス応答 / フラボノイド / プロアントシアニジン / 機能ゲノム科学

Outline of Final Research Achievements

Flavonoids have received much attention for their potential contribution to human health.　We performed the detailed analysis of the composition of each flavonoid as well as comprehensive gene expression analysis in the grape berries cultured in various environment. Genes which were coordinately expressed with proanthocyanidin (PA) accumulation under different light conditions, and a new PA regulator, VvMYBPAR, was identified. PA-specific regulation of VvMYBPAR was confirmed by VvMYBPAR constitutive expression in Arabidopsis and Grapevine as well as transient reporter assay using grapevine cells. VvMYBPAR showed a unique spatiotemporal expression during berry development as well as responce to environmental stimuli, in contrast to already identified PA regulators, suggesting the regulation of different PA-specific branch genes. This new factor suggested the polygenic regulation of PA biosynthesis in grapes by closely related MYB transcription factors.

Free Research Field

園芸科学