Functional analyses of Solanaceae genes required for resistance to potato late blight pathogen Phytophthora infestans

2012 Fiscal Year Final Research Report

• Project/Area Number: 23780041
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant pathology
• Research Institution: Nagoya University
• Principal Investigator: TAKEMOTO Daigo 名古屋大学, 生命農学研究科, 准教授 (30456587)
• Project Period (FY): 2011 – 2012
• Keywords: 病害抵抗性 / 植物病原菌 / 植物ホルモン / ファイトアレキシン

Research Abstract

Mature Nicotiana benthamiana shows strong resistance to the potato late blight pathogen Phytophthora infestans. By screening using virus-induced random gene silencing, we isolated over 30 genes required for resistance of N. benthamiana to P. infestans. Further functional analyses revealed that isolated genes are involved in various processes for plant disease resistance including the activation of defense responses after the recognition of pathogen, pro duction of anti-microbial compounds (phytoalexin), and secretion of phytoalexin at the site of pathogen attack

Research Products

• [Journal Article] Nicotiana benthamiana Calreticulin 3a is required for the ethylene-mediated production of phytoalexins and disease resistance against oomycete pathogen Phytophthora infestans (2013)
  • Author(s): Matsukawa M., Shibata Y., Ohtsu M., Mizutani A., Mori H., Wang P., Ojika M., Kawakita K., Takemoto D
  • Journal Title: Mol. Plant-Microbe Interact Volume: (in press)
  • Peer Reviewed
• [Journal Article] Proteomic analysis of S-nitrosylated proteins in potato plant (2012)
  • Author(s): Kato H., Takemoto D. and Kawakita K
  • Journal Title: Physiol Plant Volume: (in press)
  • Peer Reviewed
• [Journal Article] N-Terminal motifs in some plant disease resistance proteins function in membrane attachment and contribute to disease resistance (2012)
  • Author(s): Takemoto D., Rafiqi M., Hurley U., Lawrence G.J., Bernoux M., Hardham A.R., Ellis J.G., Dodds P.N. and Jones D.A
  • Journal Title: Mol. Plant-Microbe Interact Volume: 25 Pages: 379-392
  • Peer Reviewed
• [Journal Article] SGT1 and HSP90 are essential for age-related non-host resistance of Nicotiana benthamiana against the oomycete pathogen Phytophthora infestans (2011)
  • Author(s): Shibata Y., Kawakita K. and Takemoto D
  • Journal Title: Physiol. Mol. Plant Pathol Volume: 75 Pages: 120-128
  • Peer Reviewed
• [Presentation] Nup107-160 複合体に含まれるヌクレオポリンの病害抵抗性と病害ストレス応答性エチレン生成における役割 (2013)
  • Author(s): 大津美奈・柴田裕介・田村謙太郎・西村いくこ・小鹿一・森仁志・川北一人・竹本大吾
  • Organizer: 平成 25 年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学. 岐阜
  • Year and Date: 2013-03-27
• [Presentation] ベンサミアナタバコの SAR8.2 はジャガイモ疫病菌抵抗性に必須な内生エリシタータンパク質である (2013)
  • Author(s): 小嶋博樹・水谷安希・柴田裕介・川北一人・竹本大吾
  • Organizer: 平成 25 年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学. 岐阜
  • Year and Date: 2013-03-27
• [Presentation] ベンサミアナタバコはジャガイモ疫病菌侵入前と侵入後に活性化するテルペノイド合成経路をスイッチする (2013)
  • Author(s): 柴田裕介・小鹿一・川北一人・竹本大吾
  • Organizer: 平成 25 年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学. 岐阜
  • Year and Date: 2013-03-27
• [Presentation] ベンサミアナタバコの病害抵抗性応答誘導時のエチレン生合成における核膜孔タンパク質 NUP75 の機能解析 (2012)
  • Author(s): 大津美奈・柴田裕介・小鹿一・森仁志・川北一人・竹本大吾
  • Organizer: 平成 24 年度 日本植物病理学会関西部会
  • Place of Presentation: とりぎん文化会館. 鳥取
  • Year and Date: 2012-09-28
• [Presentation] ベンサミアナタバコの PDR1 を介した複数の抗菌性テルペノイドの分泌がジャガイモ疫病菌に対する侵入・拡大抵抗性に重要である (2012)
  • Author(s): 柴田裕介・小鹿一・川北一人 ・竹本大吾
  • Organizer: 平成 24 年度 植物感染生理談話会
  • Place of Presentation: 休暇村近江八幡. 近江八幡
  • Year and Date: 2012-08-31
• [Presentation] A nuclear pore complex protein, Nup75, is involved in ethylene biosynthesis for phytoalexin production of Nicotiana benthamiana in the defense responses against P. infestans (2012)
  • Author(s): Otsu M., Shibata Y., Ojika M., Mori H., Kawakita K. and Takemoto D
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2012-07-31
• [Presentation] NbPDR1, a PDR-type ABC transporter, confers pre- and post-invasion resistances of Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans (2012)
  • Author(s): Shibata Y., Ojika M., Kawakita K. and Takemoto D
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2012-07-31
• [Presentation] Imaging of pathogenic and symbiotic fungi in culture and during infection (2012)
  • Author(s): Takemoto D.
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan.
  • Year and Date: 2012-07-29
• [Presentation] ベンサミアナタバコのジャガイモ疫病菌抵抗性に必須な核膜孔タンパク質 NUP75 の機能解析 (2012)
  • Author(s): 大津美奈・柴田裕介・小鹿一・森仁志・川北一人・竹本大吾
  • Organizer: 平成 24 年度 日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場. 福岡
  • Year and Date: 2012-03-29
• [Presentation] ベンサミアナタバコ PDR1 を介したジャガイモ疫病菌侵入抵抗性に必須な抗菌物質の生合成系に関する研究 (2012)
  • Author(s): 柴田裕介・小鹿一・川北一人・竹本大吾
  • Organizer: 平成 24 年度 日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場. 福岡
  • Year and Date: 2012-03-29
• [Presentation] PDR-type ABC transporter contributes pre- and post-invasion resistance of Nicotiana benthamiana against Phytophthorainfestans (2012)
  • Author(s): Shibata Y., Ojika M., Kawakita K. and Takemoto D
  • Organizer: The 2nd Korea-Japan Joint Symposium, Fukuoka International Congress Center
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2012-03-27
• [Presentation] ベンサミアナタバコの疫病菌抵抗性に必須な ABC トランスポーターPDR1 は複数の抵抗反応を制御する (2012)
  • Author(s): 柴田裕介・川北一人・竹本大吾
  • Organizer: 平成 24 年度 日本植物生理学会
  • Place of Presentation: 京都産業大学. 京都
  • Year and Date: 2012-03-16
• [Presentation] ベンサミアナタバコのジャガイモ疫病菌抵抗性に必須な SAR8.2 遺伝子群の機能解析 (2011)
  • Author(s): 小嶋博樹・水谷安希・柴田裕介・川北一人・竹本大吾
  • Organizer: 平成 23 年度 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松 . 高松
  • Year and Date: 2011-10-01
• [Presentation] ベンサミアナタバコのジャガイモ疫病菌抵抗性に必須な PDR 型 ABC トランスポーターの機能解析 (2011)
  • Author(s): 柴田裕介・小鹿一・川北一人・竹本大吾
  • Organizer: 平成 23 年度 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松 . 高松
  • Year and Date: 2011-10-01
• [Presentation] ベンサミアナタバコのエリシター認識と疫病菌抵抗性におけるカルレティキュリン遺伝子 NbCRT3 の役割 (2011)
  • Author(s): 松川みずき・柴田裕介・小鹿一・川北一人・竹本大吾
  • Organizer: 平成 23 年度 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松 . 高松
  • Year and Date: 2011-10-01
• [Book] Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors (2013)
  • Author(s): Takemoto D. and Jones D.A
  • Publisher: Methods Mol. Biol(in press)
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~byori/