2012 Fiscal Year Final Research Report
Search for new effector proteins in Cladosporium fulvum
| Project/Area Number |
23780046
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
IIDA Yuichiro 独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所・野菜病害虫・品質 研究領域, 研究員 (00456609)
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| Research Collaborator |
PIERRE J. G. M. de Wit Wageningen大学, 教授
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| Project Period (FY) |
2011 – 2012
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| Keywords | 植物病原糸状菌 / トマト葉かび病 / エフェクター / Avr |
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| Research Abstract |
Leaf mold of tomato is caused by the biotrophic fungus Cladosporium fulvum which complies with the gene-for-gene system. We sequenced avirulence (Avr) genes in 123 strains collected in Japan to get information about the molecular basis of adaptation to the different Cf genes. The Avr genes of these strains contain unique mutations causing adaptation to Cf genes including (i) frameshift mutations, (ii) transposon insertions in Avr2, (iii) point mutations in Avr4 and Avr4E, and (iv) deletions of Avr4E or Avr9. Proteomics analysis of the apoplastic fluid and RNAseq data were employed to collect the candidates of new effector gene. Among them we identified Avr5 that is conferred by the tomato resistance gene Cf-5 and is located on 1.8 Mb small chromosome.
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[Presentation] New races with unique mutations in avirulence genes overcoming tomato Cf resistance genes in a Japanese population of Cladosporium fulvum2012
Author(s)
Iida Y., van't Hof P., Stergiopoulos I., Mehrabi R., Beenen H., Notsu A., Kubota M., Bahkali A., Abd-Elsalam K., Terami F., Collemare J. and de Wit P.J.G.M.
Organizer
XV International Congress on Molecular Plant-Microbe Interactions
Place of Presentation
Kyoto
Year and Date
2012-07-31
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[Presentation] Unique mutations in effector genes of a Cladosporium fulvum population in Japan have caused adaptation to different tomato Cf genes2011
Author(s)
Iida Y., van't Hof P., Stergiopoulos I., Mehrabi R., Beenen H., Notsu A., Kubota M., Bahkali A., Abd-Elsalam K., Terami F., Collemare J. and de Wit P.J.G.M.
Organizer
XIII International Congress of Mycology
Place of Presentation
Sapporo
Year and Date
2011-09-08
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