Structural and functional analyses of capsid protein and RNA polymerase in the candidate virus for honeybee colony collapse disorder

2012 Fiscal Year Final Research Report

• Project/Area Number: 23780051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied entomology
• Research Institution: Tokushima Bunri University
• Principal Investigator: HATAKEYAMA Dai 徳島文理大学, 薬学部, 講師 (20514821)
• Project Period (FY): 2011 – 2012
• Keywords: 昆虫病理

Research Abstract

Colony Collapse Disorder (CCD) is a serious problem threatening the health of honeybees and the economic stability of commercial beekeeping and pollination operations in the world. In this study, we focused on Israeli Acute Paralysis Virus (IAPV), which is considered as a candidate reason for CCD, and challenged to investigate the structure and function of the capsid protein and the RNA polymerase in IAPV. Recombinant proteins of the capsid proteins were expressed, solubilized and purified by combinational expression of 2 or 3 kinds of subunit proteins. Next, we found 2 proteins, which seemed to interact with these recombinant capsid protein subunits, from honeybee brains and guts. Two of 6 partial recombinant proteins of RNA polymerase was successful for expression and solubilization. We will try to isolate the proteins in honeybee tissues which interact with these recombinant proteins.

Research Products

• [Journal Article] Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea. (2013)
  • Author(s): Hiasa M, Kurokawa M, Ohta K, Esumi T, Akita H, Niki K, Yagi Y, Echigo N, Hatakeyama D, Kuzuhara T.
  • Journal Title: Food Research International. Volume: (In press)
• [Journal Article] Anti-influenza activity of C60 fullerene derivatives. (2013)
  • Author(s): Shoji M, Takahashi E, Hatakeyama D, Iwai Y, Morita Y, Okutani T, Echigo N, Kido H, Nakamura S, Mashino T, Kuzuhara T.
  • Journal Title: PLoS One. Volume: (In press)
• [Journal Article] Consolidation of long-term memory by insulin in Lymnaea is not brought about by changing the number of insulin receptors. (2013)
  • Author(s): Hatakeyama D, Okuta A, Otsuka E, Lukowiak K, Ito E.
  • Journal Title: Communicative & Integrative Biology Volume: 6 Pages: e23955
• [Journal Article] Involvement of insulin-like peptide in long-term synaptic plasticity and long-term memory of the pond snail Lymnaea stagnalis. (2013)
  • Author(s): Murakami J, Okada R, Sadamoto H, Kobayashi S, Mita K, Sakamoto Y, Yamagishi M, Hatakeyama D, Otsuka E, Okuta A, Sunada H, Takigami S, Sakakibara M, Fujito Y, Awaji M, Moriyama S, Lukowiak K, Ito E.
  • Journal Title: Journal of Neuroscience Volume: 33 Pages: 371-383
• [Journal Article] Inhibition of monoamine oxidase A and stimulation of behavioural activities in mice by the inactive prodrug form of the anti-influenza agent oseltamivir. (2013)
  • Author(s): Hiasa M, Isoda Y, Kishimoto Y, Saitoh K, Kimura Y, Kanai M, Shibasaki M, Hatakeyama D, Kirino Y, Kuzuhara T.
  • Journal Title: British Journal of Pharmacology Volume: 169 Pages: 115-29
• [Journal Article] Memory trace in feeding neural circuitry underlying conditioned taste aversion in Lymnaea. (2012)
  • Author(s): Ito E, Otsuka E, Hama N, Aonuma H, Okada R, Hatakeyama D, Fujito Y, Kobayashi S.
  • Journal Title: PLoS One Volume: 7 Pages: e43151
• [Journal Article] Anti-influenza activity of marchantins, macrocyclic bisbibenzyls contained in liverworts. (2011)
  • Author(s): Iwai Y, Murakami K, Gomi Y, Hashimoto T, Asakawa Y, Okuno Y, Ishikawa T, Hatakeyama D, Echigo N, Kuzuhara T.
  • Journal Title: PLoS One Volume: 6 Pages: e19825
• [Journal Article] Does conditioned taste aversion learning in the pond snail Lymnaea stagnalis produce conditioned fear? (2011)
  • Author(s): Kita S, Hashiba R, Ueki S, Kimoto Y, Abe Y, Gotoda Y, Suzuki R, Uraki E, Nara N, Kanazawa A, Hatakeyama D, Kawai R, Fujito Y, Lukowiak K, Ito E.
  • Journal Title: Biological Bulletin Volume: 220 Pages: 71-81
• [Presentation] インフルエンザウイルスRNA合成酵素における自己アセチル化能の発見 (2013)
  • Author(s): 畠山大,廣田丈典,柳澤伸,長江萌菜美,庄司正樹,葛原隆
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜市
  • Year and Date: 20130327-30
• [Presentation] インフルエンザウイルスRNA合成酵素のPB2サブユニットにおける自己アセチル化能の構造機能学的解析 (2012)
  • Author(s): 畠山大,廣田丈典,柳澤伸,長江萌菜美,庄司正樹,葛原隆
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 20121211-14
• [Presentation] インフルエンザウイルスRNA依存性RNA合成酵素のPB2サブユニットにおける自己アセチル化能の構造学的および機能学的解析 (2012)
  • Author(s): 畠山大,廣田丈典,柳澤伸,長江萌菜美,庄司正樹,葛原隆
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 20121113-15
• [Presentation] コケ植物由来の大環状ビスビベンジル分子による抗インフルエンザウイルス活性 (2011)
  • Author(s): 畠山大,岩井佑磨,村上宏起,五味康行,奥谷武史,橋本敏弘,浅川義範,奥野良信,石川豊数,越後典子,葛原隆
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 20110916-18
• [Book] Advanced Methods in Neuroethological Research (2013)
  • Author(s): Hatakeyama D, Tierling S, Kuzuhara T, Muller U.
  • Publisher: Springer.(In press)
• [Remarks] 徳島文理大学薬学部生化学教室
  • URL: http://p.bunri-u.ac.jp/lab08/index.html