Characterization of the molecular mechanism of anhydrobiosis and functional gene analysis in the chironomid Polypedilum vanderplanki.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23780055
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: CORNETTE Richard 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (20376586)
• Project Period (FY): 2011 – 2013
• Keywords: ネムリユスリカ / 乾燥耐性 / 酸化ストレス / マイクロアレイ解析 / RNAi / 培養細胞 / Cnc-C / 細胞周期

Research Abstract

Larvae of the sleeping chironomid (Polypedilum vanderplanki) can survive total desiccation in an ametabolic state known as anhydrobiosis. Gene expression during the recovery process was investigated by microarray. Whereas stress genes, such as antioxidants, were still expressed during the resuming of metabolism just after rehydration, 24 h later, we observed genes involved in the repair of biological molecules. DNA repair is tightly associated with cell cycle but synchronization of cell cycle was not detected during anhydrobiosis. On the other hand, oxidative stress induced anhydrobiosis-related gene expression. Consequently, reactive oxygen species could be the main trigger of anhydrobiosis. Thus, we characterized the transcription factor involved in oxidative stress sensing, Cnc-C, whose expression was up-regulated upon desiccation. RNAi experiments on P. vanderplanki cell cultures suggested that Cnc-C was involved in the induction of anhydrobiosis-related gene expression.

Research Products

• [Journal Article] An abundant LEA protein in the anhydrobiotic midge, PvLEA4, acts as a molecular shield by limiting growth of aggregating protein particles (2013)
  • Author(s): Hatanaka R, Hagiwara-Komoda Y, Furuki T, Kanamori Y, Fujita M, Cornette R, Sakurai M, Okuda T, Kikawada T
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 43 Pages: 1055-1087
• [Journal Article] Diversity of LEA-like proteins and its mRNA expression in the sleeping chironomids (2013)
  • Author(s): Gusev, O, Hatanaka, R, Cornette R, Kikawada T
  • Journal Title: Cryobiology and Cryotechnology Volume: 59(1) Pages: 47-50
• [Journal Article] Elucidation of mechanisms underlying desiccation tolerance with exhaustive gene analysis (2012)
  • Author(s): Mukae K, Cornette R, Gusev O, Hatanaka R, Okuda T, Kikawada T
  • Journal Title: 低温生物工学会誌 Volume: 58(1) Pages: 73-76
• [Presentation] Oxidative stress and anhydrobiosis in the desiccation-tolerant midge, Polypedilum vanderplanki (2014)
  • Author(s): Cornette R, Gusev O, Hatanaka R, Okada J, Kikawada, T, Okuda
  • Organizer: New frontiers in anhydrobiosis (Program and abstracts)
  • Year and Date: 20140000
• [Presentation] ユスリカ科における小さいゲノムサイズと極限環境に対する適応能力 (2013)
  • Author(s): Cornette R, Gusev O,志村幸子,黄川田隆洋,奥田隆
  • Organizer: 第14回極限環境生物学会年会プログラム&要旨集
  • Year and Date: 20130000
• [Presentation] Understanding the evolution of anhydrobiosis in the sleeping Chironomid, Polypedilum vanderplanki, by comparative genomics (2013)
  • Author(s): Cornette R, Gusev O, Suetsugu Y, Okuda T, Kawashima T, Sato N, Nishiyama T, Hasebe M, Kikawada T
  • Organizer: 5th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Year and Date: 20130000
• [Presentation] ネムリユスリカの乾燥耐性における酸化ストレスの影響 (2013)
  • Author(s): Cornette R,岩田健一,金森保志, Gusev O,黄川田隆洋,奥田隆
  • Organizer: 第57回日本応用動物昆虫学会大会講演要旨
  • Year and Date: 20130000
• [Presentation] ネムリユスリカの極限的な乾燥耐性 : 酸化ストレスと共に進化した現象なのか? (2012)
  • Author(s): コルネット・リシャー,グセフ・オレグ,末次克行,川島武士,佐藤矩行,黄川田隆洋
  • Organizer: 新学術領域研究「複合適応形質進化の遺伝子基盤解明」平成24年度公開シンポジウム
  • Year and Date: 20120000
• [Presentation] Oxidative stress is a major factor for the induction of anhydrobiosis in the desiccation-tolerant midge, Polypedilum vanderplanki (2012)
  • Author(s): Cornette R, Iwata K, Kanamori Y, Gusev O, Kikawada T, Okuda T
  • Organizer: XXIV International Congress of Entomology
  • Year and Date: 20120000
• [Presentation] Molecular mechanisms of anhydrobiosis in larvae of the African midge Polypedilum vanderplanki. (2012)
  • Author(s): Cornette R, Kikawada, T, Okuda, T
  • Organizer: 6th International Workshop on Desiccation Sensitivity and Tolerance in Seeds and Vegetative Plant Tissues
  • Year and Date: 20120000
• [Presentation] Adaptation to extreme environments and small genome size in Chironomids (2011)
  • Author(s): Cornette R, Gusev O, Nakahara Y, Shimura S, Kikawada T, Okuda T
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Year and Date: 20110000