2012 Fiscal Year Final Research Report
Structural analysis of plant borate transporter
Project/Area Number |
23780060
|
Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plant nutrition/Soil science
|
Research Institution | The University of Tokyo |
Principal Investigator |
KASAI Koji 東京大学, 大学院・農学生命科学研究科, 特任准教授 (80517938)
|
Project Period (FY) |
2011 – 2012
|
Keywords | 植物栄養代謝 |
Research Abstract |
We screened conditions for solubilization, purification, and crystallization of plant BOR borate transporter. We found that combination of Arabidopsis BOR4 and detergent dodecylmaltoside (DDM) showed high monodispersity. Furthermore, addition of DIDS (anion exchanger inhibitor) or borate increased thermal stability of BOR4-DDM complex.
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Research Products
(7 results)