Molecular mechanism of phosphate transport in arbuscular mycorrhiza

2013 Fiscal Year Final Research Report

• Project/Area Number: 23780062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Shinshu University
• Principal Investigator: SAITO Katsuharu 信州大学, 農学部, 准教授 (40444244)
• Project Period (FY): 2011 – 2013
• Keywords: 共生 / 菌根 / リン酸 / ポリリン酸 / 酸性ホスファターゼ / ミヤコグサ / 電子顕微鏡

Research Abstract

Phenotype of LjPAP3 knockdown in Lotus japonicus was evaluated to elucidate the molecular mechanism of phosphate transport in arbuscular mycorrhiza. The knockdown plants were defective in phosphate uptake. Arbuscular mycorrhizal fungi colonized in the knockdown plants had much more polyphosphate than in the wild-type plants. These results suggested that LjPAP3 is involved in phosphate transport via mycorrhizal symbiosis. Polyphosphate polymerases of arbuscular mycorrhizal fungus were identified: VTC1, VTC2 and VTC4. The VTC4 generated polyphosphate from ATP.

Research Products

• [Journal Article] 菌根共生からみる植物栄養の新時代 (2014)
  • Author(s): 小八重善裕,東樹宏和,山本哲史,竹本大吾,江沢辰広,河原愛,荒川竜太,齋藤勝晴,中川知己
  • Journal Title: 日本土壌肥料学雑誌 Volume: (印刷中)
• [Journal Article] 草地・耕地生態系の菌根の生理生態と利用―はじめに― (2014)
  • Author(s): 齋藤勝晴
  • Journal Title: 日本草地学会誌 Volume: 59 Pages: 261-262
• [Journal Article] Isolation and phenotypic characterization of Lotus japonicus mutants specifically defective in arbuscular mycorrhizal formation (2014)
  • Author(s): Kojima, T, K. Saito, H. Oba, Y. Yoshida, J. Terasawa, Y. Umehara, N. Suganuma, M. Kawaguchi and R. Ohtomo
  • Journal Title: Plant and Cell Physiology Volume: 55 Pages: 928-941
  • DOI: 10.1093/pcp/pcu024
  • Peer Reviewed
• [Journal Article] The genome of an arbuscular mycorrhizal fungus provides insights into the oldest plant symbiosis (2013)
  • Author(s): Tisserant, E., M. Malbreil, A. Kuo, A. Kohler, A. Symeonidi, R. Balestrini, P. Charron, N. Duensing, N. F. dit Frey, V. Gianinazzi-Pearson, B. Gilbert, Y. Handa, J. Herr, M. Hijri, R. Koul, M. Kawaguchi, F. Krajinski, P. Lammers, F. G. Masclaux, C. Murat, E. Morin, S. Ndikumana, M. Pagni, D. Petitpierre, N. Requena, P. Rosikiewicz, R. Riley, K. Saito, H. S. Clemente, H. Shapiro, D. van Tuinen, G. Bécard, P. Bonfante, U. Paszkowski, Y. Shachar-Hill, G. A. Tuskan, J. P. W. Young, I. R. Sanders, B. Henrissat, S. A. Rensing, I. V. Grigoriev, N. Corradi, C. Roux and F. Martin
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110 Pages: 20117-20122
  • DOI: 10.1073/pnas.1313452110
  • Peer Reviewed
• [Journal Article] Inorganic polyphosphates are stored in spherites within the midgut of Anticarsia gemmatalis and play a role in copper detoxification (2012)
  • Author(s): Gomes, F. M., D. B. Carvalho, A. C. Peron, K. Saito, K. Miranda and E. A. Machado
  • Journal Title: Journal of Insect Physiology Volume: 58 Pages: 211-221
  • DOI: 10.1016/j.jinsphys.2011.09.008.
• [Journal Article] アーバスキュラー菌根菌 : 研究の最前線と土壌肥料分野への貢献 (2012)
  • Author(s): 坂本一憲,秋山康紀,林英雄,磯部勝孝,江澤辰広,菊池裕介,土方野分,俵谷圭太郎,伊豆進,齋藤勝晴
  • Journal Title: 日本土壌肥料学雑誌 Volume: 83 Pages: 216-221
• [Journal Article] Acidocalcisomes as calcium- and polyphosphate-storage compartments during embryogenesis of the insect Rhodnius prolixus Stahl (2011)
  • Author(s): Ramos, I, F. Gomes, C. M. Koeller, K. Saito, N. Heise, H. Masuda, R. Docampo, W. de Souza, E. A. Machado and K. Miranda
  • Journal Title: PLoS One Volume: 6 Pages: 27276
  • DOI: 10.1371/journal.pone.0027276
• [Journal Article] アーバスキュラー菌根菌との共生 (2011)
  • Author(s): 齋藤勝晴,林誠
  • Journal Title: 細胞工学 Volume: 30 Pages: 149-154
• [Journal Article] Vacuolar pH of living hyphae of an arbuscular mycorrhizal fungus
  • Author(s): Funamoto R, K. Saito, H. Oyaizu, T. Aono and M. Saito
  • Journal Title: Gigaspora margarita Volume: (in press)
  • DOI: 10.1007/s00572-014-0588-1
  • Peer Reviewed
• [Presentation] LjPAP3 encoding purple acid phosphatase involved in P-transfer in the plant-fungal interface of arbuscular mycorrhiza (2013)
  • Author(s): Saito, K, Osada, Y, Nakiri, K, Saito, M. and Ezawa, T
  • Organizer: 7th International Conference on Mycorrhiza (ICOM7)
  • Place of Presentation: India Habitat Centre. New Delhi, India.
  • Year and Date: 20130106-11
• [Presentation] Localization of polyphosphate in arbuscular mycorrhizal fungus colonizing in Lotus japonicus (2012)
  • Author(s): Saito, K., Osada, Y, Nakiri, K., Nishimura, A, Matsumoto, C, Saito, M. and Ezawa, T
  • Organizer: International Society for Molecular Plant-Microbe Interactions 2012 XV International Congress
  • Place of Presentation: Kyoto
  • Year and Date: 20120729-0802
• [Presentation] 菌根誘導性パープル酸性ホスファターゼのRNAi系統を用いたポリリン酸局在解析 (2012)
  • Author(s): 齋藤勝晴,名切孝介,松本忠太,齋藤雅典,江沢辰広
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取大会
  • Year and Date: 2012-09-04
• [Presentation] 菌根共生で誘導されるパープル酸性ホスファターゼLjPAP3の機能解析 (2011)
  • Author(s): 齋藤勝晴,長田泰幸,西村あおい,上原洋平,名切孝介,間瀬ひろみ,松本忠大,齋藤雅典,江沢辰広
  • Organizer: 菌根研究会
  • Place of Presentation: 広島大学
  • Year and Date: 2011-12-10
• [Presentation] 菌根の樹枝状体におけるポリリン酸局在と酸性ホスファターゼ活性の相関 (2011)
  • Author(s): 長田泰幸,名切孝介,斎藤勝晴
  • Organizer: 植物微生物研究会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-20
• [Presentation] 菌根共生で誘導されるパープル酸性ホスファターゼLjPAP3の局在解析 (2011)
  • Author(s): 上原洋平,江沢辰広,齋藤勝晴
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: つくば
  • Year and Date: 2011-08-08