2012 Fiscal Year Final Research Report
A novel oxidative stressdefense mechanism of Escherichia coliby cysteine/cystine shuttle system play a important role in quality control of membrane phospholipids
| Project/Area Number |
23780081
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
OHTSU Iwao 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60395655)
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| Project Period (FY) |
2011 – 2012
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| Keywords | システイン / ペリプラズム / 大腸菌 / 過酸化脂質 / 酸化ストレス |
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| Research Abstract |
The inducible L-cysteine/L-cystine shuttle system plays an important role on quality control of lipids under oxidative stress. Thiobarbituric acid responsive substances (TBARS) are routinely used to assess oxidative stress damage to membrane lipids in diverse organisms.TBAR content increased ~2-fold when E. coliwas exposed to H2O2, respectively. Even in the absence of toxicant, E. coli ΔydeD/fliYshowed increased (~2-fold) levels of these substances relative to wild-type controls, suggesting that L-cysteine/L-cystine shuttle systemmay function in controlling the level of membrane peroxidation products thatare generated during the normal, basal metabolism.
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