2012 Fiscal Year Final Research Report
Functional analysis of novel transporter involved in intra-cellular transport of nicotine biosynthetic intermediates.
| Project/Area Number |
23780109
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 植物 / アルカロイド / トランスポーター / ニコチン / 転流 |
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| Research Abstract |
Plants produce a large number of secondary metabolites. Some metabolites are often highly accumulated in particular organs of medicinal plants. The movement of biosynthetic intermediates within the organelles in a cell is also suggested. However, little is known how metabolites are transported across membranes and how these movements are involved in metabolite production. Tobacco T408 is a plastid-localized transporter which might regulate intermediate transport in a cell. We altered expression level of T408 by producing stable transformants of tobacco plants and cultured cells. Transgenic cultured cells produced higher level of alkaloids than control cells under jasmonate treatment. These data suggested that productions of secondary metabolites are increased by regulating the expression level of specific transporter in a cell.
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