2012 Fiscal Year Final Research Report
Analysis ofthe relationship between tchoenformational stabilityof phospholipaseA2 and the amount of IgG production.
| Project/Area Number |
23780110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Sojo University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | タンパク質工学 |
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| Research Abstract |
To examin relationship between the conformational stability of phospholipaseA2 isozyme (BPII) and the amount of IgG production, I prepared the destabilized BPII where the N-terminal region (amino acid position 1-8) was deleted by BrCN treatment (BrCN-BPII). As a result, BrCN-BPII could not lead to increase in the immune response. On the other hand, I examined the cell death activity of BPII against C2C12 cells (mouse myoblast cell line). When C2C12 cells were treated with intact BPII, the viability of the cells decreased. Interestingly, BrCN-BPII could not induce the cell death. It was suggested that the N-terminal region of BPII is an essential element for its cell death.
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