2013 Fiscal Year Final Research Report
Searching for dietary protein/peptide sensing receptors in enteroendocrine cells
| Project/Area Number |
23780124
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Hokkaido University |
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Principal Investigator |
HIRA Tohru 北海道大学, (連合)農学研究科(研究院), 講師 (10396301)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 消化管内分泌細胞 / 栄養素受容体 / 食品タンパク質 / 食品ペプチド |
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| Research Abstract |
We searched sensor molecules involved in dietary protein/peptide-sensing in enteroendocrine cells. By using enteroendocrine cell models, we found that a TRP channel was highly expressed in CCK-producing cell line.
It was demonstrated that the TRP channel is involved in alpha-casein-induced CCK secretion. Furthermore, an aldehyde was found to induce CCK secretion through the TRP channel. Oral administration of the aldehyde reduced gastric emptying rate in rats, suggesting the aldehyde directly acts enteroendocrine cell and reduces gastric emptying in vivo.
In separated experiments, we found that calcium-sensing receptor functions as sensor for various protein hydrolysates, rather than for specific peptides.
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