Anti-cancer effect by metabolic conversion

2012 Fiscal Year Final Research Report

• Project/Area Number: 23780132
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Nagoya University
• Principal Investigator: KITAURA Yasuyuki 名古屋大学, 生命農学研究科, 助教 (90442954)
• Project Period (FY): 2011 – 2012
• Keywords: がん / 脂肪酸

Research Abstract

Shifting unique metabolism in cancer cells from glycolysis to glucose oxidation can decrease their cell viabilities. We investigated whether altering branched-chain amino acid (BCAA) metabolism also killed cancer cells. Medium-chain fatty acid, caprylate (C8) which activates BCAA metabolism induced cell death of cancer cells with no effect on normal cells. We found that the most effective MCFA was laurate (C12) which had no effect on BCAA metabolism. The treatment of laurate with oleate dramatically decreased the cell viabilities of several cancer cells. Laurate increased the uptake of oleate and the excess amount of intracellular oleate that induce cell death by lipotoxic stress response, which vary by cell type. Moreover, intraperitoneal administration of laurate with unsaturated fatty acid to Ehrich ascite tumor-bearing mice significantly inhibited the accumulation of ascitic fluid in the peritoneal cavities and increased the survival rate of mice. These results suggest that the combination of laurate and unsaturated fatty acid has a great potential for the anti-tumor activity.

Research Products

• [Presentation] The effect of combination of Lauric and Oleic acids on lipotoxicity (2013)
  • Author(s): Yasuyuki Kitaura, Kana Inoue, Naoki Kato, Nahomi Matsushita, Yoshiharu shimomura
  • Organizer: IUNS 20th International Congress of Nutrition
  • Place of Presentation: グラナダ(スペイン)
  • Year and Date: 20130900
• [Presentation] ラウリン酸と多価不飽和脂肪酸との組み合わせによる癌抑制効果 (2013)
  • Author(s): 河東 直規、井上 花菜、松下 なほみ、北浦 靖之、下村 吉治
  • Organizer: 第67回日本・栄養食糧学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20130500
• [Presentation] Oleate promotes laurate-induced apoptosis of cancer cells (2011)
  • Author(s): Yasuyuki Kitaura, Kana Inoue, Toshiaki Aoyama, Yoshiharu shimomura
  • Organizer: The XI Asian Congress of Nutrition (ACN)
  • Place of Presentation: シンガポール
  • Year and Date: 20110700
• [Presentation] ラウリン酸による癌細胞の細胞死誘導効果 (2011)
  • Author(s): 井上 花菜、北浦 靖之、青山 敏明、下村 吉治
  • Organizer: 第65回日本・栄養食糧学会大会
  • Place of Presentation: 東京
  • Year and Date: 20110500
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~nutr/shimomura/yan_jiugurupuTop.html
• [Remarks] 名古屋大学新技術説明会 2011年11月・東京
• [Remarks] 異なる脂肪酸を組み合わせることで強力にがん細胞を死滅させる
• [Remarks] 第4回名古屋大学医学・バイオ系知財フェア 2011年 12月・名古屋
• [Remarks] 中鎖脂肪酸と不飽和脂肪酸による癌細胞死誘導に対する相乗効果
• [Remarks] 日刊工業新聞 2012年1月20日
• [Remarks] 中部地区医療・バイオ系シーズ発表会 2012年12月・名古屋
• [Remarks] 異種脂肪酸の組み合せによる癌細胞死誘導効果
• [Patent(Industrial Property Rights)] 細胞死誘導効果を有する中鎖飽和脂肪酸と不飽和脂肪酸を含有する組成物 (2011)
  • Inventor(s): 下村 吉治、北浦 靖之、井上 花菜
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Number: 特許出願2011-93212
  • Filing Date: 2011-04-19