2012 Fiscal Year Final Research Report
The analytical method of post-translational modification to proteins related to the neurodegenerative disorder.
| Project/Area Number |
23780147
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Meijo University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 翻訳後修飾 / 脂質過酸化 / モノクローナル抗体 / ELISA / 酸化ストレス |
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| Research Abstract |
In this study, the monoclonal antibodies, which could recognize the propanoylation, one of non-enzymatic post-translational modification derived from lipid peroxidation products in addition to amino acid sequences of the target protein, were obtained to construct the quantification method of biomarker related to the neurodegenerative disorder. We constructed the competitive ELISA using this antibody and prepared the standard curve. These results suggested that it was possible to quantify the level of this modification in vivo as the biomarker.
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