2013 Fiscal Year Final Research Report

Development of a novel simultaneous detection and identification method for genetically modified plants using DNA ID tag

Research Project

Project/Area Number	23780151
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Food science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	TAKABATAKE Reona 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品分析研究領域, 主任研究員 (20463466)
Project Period (FY)	2011 – 2013
Keywords	食品分析
Research Abstract	The number of GM crops has been continuously increasing, and it is virtually impossible to detect comprehensively all of these crops. As a pilot study, we generated GM plants which was introduced several DNA ID tags, and several rice transgenic lines were obtained. We evaluated the system using extracted rice gnomic DNA and designed common primers and specific probes. It was confirmed that the introduced ID tags were detectable with conventional PCR analysis using the common primer pair and the three DNA ID tags were identified with real-time PCR analysis using specific DNA probes which were designed in the ID tag regions.

(2 results)