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2013 Fiscal Year Final Research Report

Elucidation of the selective lignin degradation mechanism in a white rot fungus and the biosynthetic pathway of its key metabolites through a proteomic approach

Research Project

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Project/Area Number 23780183
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionKyoto University

Principal Investigator

WATANABE Takahito  京都大学, 生存圏研究所, 助教 (30362403)

Project Period (FY) 2011 – 2013
Keywordsリグニン / 白色腐朽菌 / 脂質関連代謝物 / プロテオーム
Research Abstract

A white-rot fungus, Ceriporiospsis subvermispora, can selectively degrade lignin without serious damage to cellulose. In order to elucidate the molecular mechanism of the selective lignin degradation, we tried to investigate the cellular response of C. subvermispora to a lignin fragment, vanillin. We obtained expression profiles of C. subvermispora in the presence and absence of vanillin through a proteomics-based approach, and detected vanillin-induced and repressed proteins. Using suppression subtractive hybridization, moreover, we also obtained many genes clones that are up-regulated in the presence of vanillin.

  • Research Products

    (6 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Vanillin-induced cellular response in the white rot fungus, Ceriporiopsis subvermispora2013

    • Author(s)
      Watanabe, T., Nishimura, H, Watanabe, T
    • Journal Title

      Sustainable Humanosphere

      Volume: 9 Pages: 2

    • URL

      http://www.rish.kyoto-u.ac.jp/ISSN/1880-6503/

  • [Journal Article] Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis2012

    • Author(s)
      Fernandez-Fueyo, E., Ruiz-Duenas, F. J., Ferreira, P., Floudas, D., Hibbett, D. S., Canessa, P., Larrondo, L. F., James, T. Y., Seelenfreund, D., Lobos, S., Polanco, R., Tello, M., Honda, Y., Watanabe, T., Watanabe, T., Ryu, J. S., Kubicek, C. P., Schmoll, M., Gaskell, J., Hammel, K. E., St John, F. J., Vanden Wymelenberg, A., Sabat, G., Splinter BonDurant, S., Syed, K., Yadav, J. S., Doddapaneni, H., Subramanian, V., Lavin, J. L., Oguiza, J. A., Perez, G., Pisabarro, A. G., Ramirez, L., Santoyo, F., Master, E., Coutinho, P. M., Henrissat, B., Lombard, V., Magnuson, J. K., Kues, U., Hori, C., Igarashi, K., Samejima, M., Held, B. W., Barry, K. W., LaButti, K. M., Lapidus, A., Lindquist, E. A., Lucas, S. M., Riley, R., Salamov, A. A., Hoffmeister, D., Schwenk, D., Hadar, Y., Yarden, O., de Vries, R. P., Wiebenga, A., Stenlid, J., Eastwood, D., Grigoriev, I. V., Berka, R. M., Blanchette, R. A., Kersten, P., Martinez, A. T., Vicuna, R., Cullen, D
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A

      Volume: 109 Pages: 5458-5463

    • DOI

      10.1073/pnas.1119912109

    • Peer Reviewed
  • [Presentation] 選択的白色腐朽菌Ceriporiopsis subvermisporaのバニリンに対する細胞応答2013

    • Author(s)
      渡邊崇人,森賢一郎,木戸彩子,長谷川隆大,西村裕志,本田与一,渡辺隆司
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば
    • Year and Date
      2013-11-21
  • [Presentation] 選択的リグニン分解菌のバニリンに対する細胞応答2013

    • Author(s)
      渡邊崇人,森賢一郎,木戸彩子,長谷川隆大,和泉千尋,津田冴子,西村裕志,渡辺隆司
    • Organizer
      日本農芸化学会2013年度(平成25年度)大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
  • [Presentation] Cellular response of a selective lignin-degrading fungus to vanillin. Lignobiotech II Symposium2012

    • Author(s)
      Watanabe, T., Mori, K., Kido, A., Izumi, C., Hasegawa, T., Tsuda, S., Nishimura, H., Honda, Y., Watanabe, T
    • Organizer
      2nd Symposium on Biotechnology Applied to Ligonocelluloses
    • Place of Presentation
      Fukuoka
    • Year and Date
      20121014-17
  • [Presentation] 選択的白色腐朽菌Ceriporiopsis subvermisporaのリグニン分解フラグメントに対する転写応答及び代謝物解析2012

    • Author(s)
      森賢一郎,高田理江,渡邊崇人,本田与一,渡辺隆司
    • Organizer
      第62回日本木材学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-17

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Published: 2015-06-25  

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