2012 Fiscal Year Final Research Report
Study on the formation of two polymorphs of cellulose I in Oikopleura
| Project/Area Number |
23780191
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | 沖縄科学技術大学院大学 |
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Principal Investigator |
NAKASHIMA Keisuke 沖縄科学技術大学院大学, マリンゲノミックスユニット, 研究員 (10422924)
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| Project Period (FY) |
2011 – 2012
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| Keywords | セルロース |
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| Research Abstract |
To understand molecular mechanisms behind crystallization processes in cellulose biosynthesis, we analyzed the two cellulose synthases in the marine chordate Oikopleura dioica, those are proposed to be involved in the formation of two polymorphs of cellulose I. With negative results in assessing the embryos injected with mRNAs encoding mutated cellulose synthases, we challenged to express native forms using heterologous system with bacterial, yeast, and insect cell hosts.
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