2012 Fiscal Year Final Research Report
Establishmentof fish disease diagnosis using cytokinenetwork
| Project/Area Number |
23780199
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | University of Miyazaki |
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Principal Investigator |
KONO Tomoya 宮崎大学, IR 推進機構, 助教 (60527547)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 魚病 / サイトカイン |
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| Research Abstract |
The multiplex RT-PCR assay targeting 19 cytokine genes was constructed and the expression of cytokine genes in Fuguin response to immunostimulants or pathogenic bacteria infection was analyzed using this method. As results, increased expressionof pro-inflammatory cytokines was observed in all the experiments. Moreover, the increase in I-IFN gene expression was confirmed only by stimulation with viral mimic polyI:C. Next, production of IL-10, IL-17A/F1, IFN-y and IL-4/13 was analyzed in the kidney of Fuguinfected with pathogenic bacteria or parasite. The production of all cytokines analyzed was increased by the bacterial infection. Although similar production pattern was confirmed in parasite infection also, especially a conspicuous increase in IL-4/13 level was recorded. Next, the detection method for intracellular cytokine was established and it enabled us to detect IFN-y producing cells. Using this method, we found the increased number of IFN-y producing cells in peripheral blood leucocytes in the stimulation with bacterial component.
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[Journal Article] Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor2012
Author(s)
Wang T., Kono T., Montea M.M., Kuse H., Costa M.M., Korenaga H., Maehra T., Husaina M., Sakai M., SecombesC.J
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Journal Title
Molecular Immunology
Volume: 53
Pages: 398-409
Peer Reviewed
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