2012 Fiscal Year Final Research Report
The Development of acceleration of nuclear reprogramming by fusion with transactivation domain
| Project/Area Number |
23780281
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied animal science
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| Research Institution | Kinki University |
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Principal Investigator |
TANI Tetsuya 近畿大学, 農学部, 講師 (70319763)
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| Project Period (FY) |
2011 – 2012
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| Keywords | リプログラミング / iPS細胞 / 体細胞クローン / 転写活性化領域 |
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| Research Abstract |
In this research, we found that transcription factor-base reprogramming require strong Oct4 binding to the promoter region. We succeed strong binding Oct4 promoter region and radical acceleration of mouse iPS cell production by chimeric Oct4 with transactivation domain from MyoD and VP16. However, oocyte-base reprogramming by somatic cell nuclear transfer could not accelerated by chimeric Oct4 injection into ooplasm.
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