2012 Fiscal Year Final Research Report
Analysis of Ebola virus nucleocapsid formation
| Project/Area Number |
23780309
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NODA Takeshi 東京大学, 医科学研究所, 准教授 (00422410)
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| Project Period (FY) |
2011 – 2012
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| Keywords | エボラウイルス / ヌクレオカプシド |
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| Research Abstract |
Ebola virus VP35 is a cofactor of the viral RNA polymerase complex and, together with NP and VP24, is an essential component for nucleocapsid formation. In the present study, we examined the interactions between VP35 and NP and found that VP35 interacts with helical NP-RNA complexes through the C-terminus of NP. We also found that coexpression of excess VP35 with NP reduced the yields of NP-RNA complexes purified by CsCl gradient ultracentrifugation and inhibited the formation of the NP-induced inclusion bodies that typically form in Ebola virus-infected cells. These findings suggest that the NP to VP35 ratio is important in the Ebola virus replication cycleand advance our knowledge of nucleocapsid morphogenesis.
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[Journal Article] Inhibition of Marburg virus budding by nonneutralizing antibodies to the envelope glycoprotein2012
Author(s)
Kajihara M, Marzi A, Nakayama E, Noda T, Kuroda M, Manzoor R, Matsuno K, Feldmann H, Yoshida R, Kawaoka Y, Takada A
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Journal Title
J. Virol
Volume: 86
Pages: 13467-74
Peer Reviewed
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