2013 Fiscal Year Final Research Report
Study on the pathogenesis of canine inflammatory bowel disease: investigation of the intestinal mucosal barrier function and intestinal mucosal cytokine expression.
| Project/Area Number |
23780315
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Clinical veterinary science
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
OHTA Hiroshi 北海道大学, (連合)獣医学研究科, 助教 (50431333)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 犬 / 腸炎 / サイトカイン / インターロイキン－１７ / 粘膜バリア |
|---|
| Research Abstract |
We examined the expression of tight junction and adherens junction protein in duodenal mucosa sample of dogs with inflammatory bowel disease (IBD). The expression of TJ proteins (claudin-1, -2, -3, -4, -5, -7 and -8), and AJ proteins (E-cadherin and beta-catenin) were determined by means of immunoblotting. As a result, expression of E-cadherin protein was significantly lower in duodenal mucosa samples of dogs with IBD than it was in samples obtained from healthy control dogs. We speculated that T helper cells have important roles in change in expression of E-cadherin in duodenal mucosa of IBD dogs. Thus, we evaluated the gene expression of cytokines of T cell subsets (IL-17, IFN-gamma, IL-4, and IL-10) in duodenal mucosa from dogs with IBD. As a result, there was no significant difference in each cytokine mRNA transcription level between groups. Thus, there is no clear evidence for the involvement of distinct T helper cytokine in the pathogenesis of canine IBD.
|
Research Products
(13 results)
