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2013 Fiscal Year Final Research Report

Biosynthesis mechanisms of the the plant cell wall polysaccharides

Research Project

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Project/Area Number 23780339
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Boundary agriculture
Research InstitutionUniversity of the Ryukyus

Principal Investigator

KONISHI TERUKO  琉球大学, 農学部, 准教授 (30433098)

Project Period (FY) 2011 – 2013
Keywords植物 / 細胞壁 / 糖鎖 / 生合成 / クラミドモナス
Research Abstract

Plant cell wall polysaccharides are useful in our daily life as a source of pulp and dietary fiber. The plant cell wall polysaccharides are also considered as important source of biofuel such as bioethanol. However, the mechanism of plant cell wall biosyntehsis has been unknown. In this project, we studied UDP-arabinopyranose mutase (UAM) and UDP-galactopyranose mutase (UGM) that are the enzymes involved in the cell wall biosynthesis in order to clear the mechanism of plant cell wall biosynthesis. Japan has a lot of species of algae which possess an ability of CO2 fixation higher than land plants do and become a source of bioenergy. Thus, we focused on algae cell wall, and used Chlamydomonas reinhardtii as a smple in this project. In order to clarify the function of these enzymes in the Chlamydomonas cell, we analyzed the characterization of Chlamydomonas UAM and detected Chlamydomonas UGM activity. We also analyzed the UAM and UGM gene expression on cell cycle.

  • Research Products

    (6 results)

All 2014 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Purification and characterrri zation of UDP-arabinopyranose mutase from Chlamydomonas reinhardtii2013

    • Author(s)
      Ayana Kotani, Maki Tsuji, Yasushi Azama, Tadashi Ishii, Takumi Takeda, Tetsuro Yamashita, Mie Shimojima, and Teruko Konishi
    • Journal Title

      Biosci. Biote chnol. Biochem.

      Volume: 77; (9) Oct Pages: 1874-1878

    • Peer Reviewed
  • [Journal Article] The Origin and functions of arabinofuranosyl residues in plant cell walls2012

    • Author(s)
      Teruko Konishi and Tadashi Ishii
    • Journal Title

      Trends Glycosci. Glycotechnol

      Volume: 24 Pages: 13-23

    • Peer Reviewed
  • [Presentation] クラミドモナス細胞周期における糖ヌクレオチド合成酵素遺伝子群の解析2014

    • Author(s)
      前田美奈子(琉球大)、小西照子(琉球大)
    • Organizer
      第55回日本植物生理学会大会
    • Place of Presentation
      富山
    • Year and Date
      20140300
  • [Presentation] Prufication of Chlamydomonas UDP-arabino pyranose mutase2011

    • Author(s)
      Ayana Kotani (University of the Ryukyus), Tadashi Ishii (FFPRI), Masakuni Tako (University of the Ryukyus), and Teruko Konishi (University of the Ryukyus)
    • Organizer
      4th Cell Wall Biosynthesis Meeting
    • Place of Presentation
      Awaji
    • Year and Date
      20111000
  • [Presentation] クラミドモナスにおける糖ヌクレオチド合成酵素の特性について2011

    • Author(s)
      小谷彩奈(琉球大)、安座間康(琉球大)、石井忠(森林総研)、小西照子(琉球大)
    • Organizer
      2011クラミドモナスワークショップ
    • Place of Presentation
      岡崎(愛知)
    • Year and Date
      20110300
  • [Book] 植物由来の細胞壁分解酵素,バイオマステクノロジーシリーズ「バイオマス分解酵素の研究の最前線」2012

    • Author(s)
      小西照子,竹田匠(監修:近藤昭彦, 天野良彦, 田丸浩)
    • Publisher
      シーエムシー出版

URL: 

Published: 2015-06-25  

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