2012 Fiscal Year Final Research Report
Imaging of membrane dynamics in specialized differentiated cells by multiphoton super-resolution microscopy
| Project/Area Number |
23790039
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
HIBI Terumasa 北海道大学, 電子科学研究所, 助教 (50554292)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 破骨細胞 / 脂肪細胞 / 小胞輸送 / 蛍光タンパク質 / 多光子顕微鏡 |
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| Research Abstract |
In human body, homeostasis is maintained by functions of various specialized differentiated cells. Most of functions are due to vesicular trafficking and membrane fusion. In this work, vesicular trafficking in osteoclasts and adipocytes was analyzed with multiphoton fluorescent 4D microscopy. Additionally, combination of multiphoton microscopy with our previously developed liquid crystal devices realized both high lateral resolution and deep depth-of-field.
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