Intravital FRET Monitoring for Systematic Evaluation of Drug Delivery Systems

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: MATSUMOTO Yu 東京大学, 医学部附属病院, 助教 (80548553)
• Project Period (FY): 2011 – 2013
• Keywords: ドラッグデリバリー / 生体イメージング

Research Abstract

Efficient drug delivery system (DDS) must fulfill paradoxical requirements as it should secure the cargo in the off-target environment, and also release the cargo at the target cells. Using a combination of Forster resonance energy transfer (FRET) and intravital imaging techniques, the intravital distribution and intactness of the DDS was evaluated. Intravital performance of the DDS was assessed in many aspects such as blood circulation studies, tissue distribution, intracellular stability, and release of the nucleic acid.

Research Products

• [Journal Article] An Injectable Spheroid System with Genetic Modification for Cell Transplantation Therapy (2014)
  • Author(s): Uchida S, Itaka K, Nomoto T, Endo T, Matsumoto Y, Ishii T, Kataoka K
  • Journal Title: Biomaterials Volume: 35 Pages: 2499-506
  • Peer Reviewed
• [Journal Article] Multicompartment Micelles with Adjustable Poly(Ethylene Glycol) Shell for Efficient in Vivo Photodynamic Therapy (2014)
  • Author(s): Synatschke CV, Nomoto T, Cabral H, Fortsch M, Toh K, Matsumoto Y, Miyazaki K, Hanisch A, Schacher FH, Kishimura A, Nishiyama N, Muller AH, Kataoka K
  • Journal Title: ACS Nano Volume: 8 Pages: 1161-72
  • Peer Reviewed
• [Journal Article] Aggregation Behavior of Cationic Nanohydrogel Particles in Human Blood Serum (2014)
  • Author(s): Nuhn L, Gietzen S, Mohr K, Fischer K, Toh K, Miyata K, Matsumoto Y, Kataoka K, Schmidt M, Zentel R
  • Journal Title: Biomacromolecules
  • Peer Reviewed
• [Journal Article] Three-Layered Polyplex Micelle as a Multifunctional Nanocarrier Platform for Light-Induced Systemic Gene Transfer (2014)
  • Author(s): Nomoto T, Fukushima S, Kumagai M, Machitani K, Arnida, Matsumoto Y, Oba M, Miyata K, Osada K, Nishiyama N, Kataoka K
  • Journal Title: Nat Commun Volume: 5 Pages: 3545
  • Peer Reviewed
• [Journal Article] Targeted Gene Delivery by Polyplex Micelles with Crowded PEG Palisade and cRGD Moiety for Systemic Treatment of Pancreatic (2014)
  • Author(s): Tumors, Ge Z, Chen Q, Osada K, Liu X, Tockary TA, Uchida S, Dirisala A, Ishii T, Nomoto T, Toh K, Matsumoto Y, Oba M, Kano MR, Itaka K, Kataoka K
  • Journal Title: Biomaterials Volume: 35 Pages: 316-26
  • Peer Reviewed
• [Journal Article] 他領域からのトピックスナノバイオテクノロジーを応用した医療イノベーション (2013)
  • Author(s): 松本有,山岨達也,片岡一則
  • Journal Title: 日本耳鼻咽喉科学会会報 Volume: 113 Pages: 133-139
• [Journal Article] A Toolbox of Differently Sized and Labeled PMMA Nanoparticles for Cellular Uptake Investigations (2013)
  • Author(s): Vollrath A, Schallon A, Pietsch C, Schubert S, Nomoto T, Matsumoto Y, Kataoka K, Schubert US
  • Journal Title: Soft Matter Volume: 9 Pages: 99-108
  • Peer Reviewed
• [Journal Article] Tethered PEG Crowdedness Determining Shape and Blood Circulation Profile of Polyplex Micelle Gene Carriers (2013)
  • Author(s): Tockary TA, Osada K, Chen Q, Machitani K, Dirisala A, Uchida S, Nomoto T, Toh K, Matsumoto Y, Itaka K, Nitta K, Nagayama K, Kataoka K
  • Journal Title: Macromolecules Volume: 46 Pages: 6585-6592
  • Peer Reviewed
• [Journal Article] Cyclic RGD-Linked Polymeric Micelles for Targeted Delivery of Platinum Anticancer Drugs to Glioblastoma through the Blood-Brain Tumor Barrier (2013)
  • Author(s): Miura Y, Takenaka T, Toh K, Wu S, Nishihara H, Kano MR, Ino Y, Nomoto T, Matsumoto Y, Koyama H, Cabral H, Nishiyama N, Kataoka K
  • Journal Title: ACS Nano Volume: 7 Pages: 8583-92
  • Peer Reviewed
• [Journal Article] Lipid Envelope-Type Nanoparticle Incorporating a Multifunctional Peptide for Systemic SiRNA Delivery to the Pulmonary Endothelium (2013)
  • Author(s): Kusumoto K, Akita H, Ishitsuka T, Matsumoto Y, Nomoto T, Furukawa R, El-Sayed A, Hatakeyama H, Kajimoto K, Yamada Y, Kataoka K, Harashima H
  • Journal Title: ACS Nano Volume: 7 Pages: 73534-41
  • Peer Reviewed
• [Journal Article] Multifunctional Polyion Complex Micelle Featuring Enhanced Stability, Targetability, and Endosome Escapability for Systemic SiRNA Delivery to Subcutaneous Model of Lung Cancer (2013)
  • Author(s): Kim HJ, Ishii T, Zheng M, Watanabe S, Toh K, Matsumoto Y, Nishiyama N, Miyata K, Kataoka K, Drug Delivery and Translational
  • Journal Title: Research Volume: 4 Pages: 50-60
  • Peer Reviewed
• [Journal Article] リアルタイム生体内共焦点レーザ顕微鏡を用いたDrug Delivery Systems(DDS)の動態評価法 (2012)
  • Author(s): 野本貴大,松本有,藤加珠子, Christie RJ,宮田完二郎,大庭誠, Cabral H,村上真美,福島重人,西山伸宏,片岡一則
  • Journal Title: 薬学雑誌 Volume: 132 Pages: 1347-54
• [Journal Article] PEG-Detachable Cationic Polyaspartamide Derivatives Bearing Stearoyl Moieties for Systemic SiRNA Delivery toward Subcutaneous BxPC3 Pancreatic Tumor (2012)
  • Author(s): Kim HJ, Oba M, Pittella F, Nomoto T, Cabral H, Matsumoto Y, Miyata K, Nishiyama N, Kataoka K
  • Journal Title: J Drug Target Volume: 20 Pages: 33-42
  • Peer Reviewed
• [Journal Article] Targeted Polymeric Micelles for SiRNA Treatment of Experimental Cancer by Intravenous Injection (2012)
  • Author(s): Christie RJ, Matsumoto Y, Miyata K, Nomoto T, Fukushima S, Osada K, Halnaut J, Pittella F, Kim HJ, Nishiyama N, Kataoka K
  • Journal Title: ACS Nano Volume: 6 Pages: 5174-89
  • Peer Reviewed
• [Journal Article] Micellization of Cisplatin (NC-6004) Reduces its Ototoxicity in Guinea Pigs (2012)
  • Author(s): Baba M, Matsumoto Y, Kashio A, Cabral H, Nishiyama N, Kataoka K, Yamasoba T
  • Journal Title: J Control Release Volume: 157 Pages: 112-7
  • Peer Reviewed
• [Journal Article] In Situ Quantitative Monitoring of Polyplexes and Polyplex Micelles in the Blood Circulation Using Intravital Real-Time Confocal Laser Scanning Microscopy (2011)
  • Author(s): Nomoto T, Matsumoto Y, Miyata K, Oba M, Fukushima S, Nishiyama N, Yamasoba T, Kataoka K
  • Journal Title: J Control Release Volume: 151 Pages: 104-9
  • Peer Reviewed
• [Journal Article] I mproving Drug Potency and Efficacy by Nanocarrier-Mediated Subcellular Targeting (2011)
  • Author(s): Murakami M, Cabral H, Matsumoto Y, Wu S, Kano MR, Yamori T, Nishiyama N, Kataoka K
  • Journal Title: Sci Transl Med Volume: 3 Pages: 64ra2
  • Peer Reviewed
• [Journal Article] Effect of Polymer Structure on Micelles Formed between Sirna and Cationic Block Copolymer Comprising Thiols and Amidines (2011)
  • Author(s): Christie RJ, Miyata K, Matsumoto Y, Nomoto T, Menasco D, Lai TC, Pennisi M, Osada K, Fukushima S, Nishiyama N, Yamasaki Y, Kataoka K
  • Journal Title: Biomacromolecules Volume: 12 Pages: 3174-85
  • Peer Reviewed
• [Journal Article] Accumulation of Sub-100 Nm Polymeric Micelles in Poorly Permeable Tumours Depends on Size (2011)
  • Author(s): Cabral H, Matsumoto Y, Mizuno K, Chen Q, Murakami M, Kimura M, Terada Y, Kano MR, Miyazono K, Uesaka M, Nishiyama N, Kataoka K
  • Journal Title: Nat Nanotechnol Volume: 6 Pages: 815-23
  • Peer Reviewed
• [Presentation] Intravital Confocal Microscopy Assay for the Evaluation of Antioxidant Capacity (2014)
  • Author(s): Matsumoto Y
  • Organizer: 37th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: Manchester Grand Hyatt Hotel, San Diego, CA, USA
  • Year and Date: 2014-02-24
• [Presentation] Intravital Microscopy for DDS Evaluation and Development (2013)
  • Author(s): Matsumoto Y
  • Organizer: The 12th US-Japan Symposium on Drug Delivery Systems
  • Place of Presentation: The Westin Maui Resort and Spa, Lahaina, Maui, HI, USA
  • Year and Date: 20131216-20
• [Presentation] DDS Evaluation Using Intravital Real-Time Laser Scanning Confocal Microscopy (2013)
  • Author(s): Matsumoto Y
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hawaii Convention Center, Honolulu, HI, USA
  • Year and Date: 20130721-24
• [Presentation] Intravital Imaging of Drug Delivery Systems and Auditory Hair Cells (2013)
  • Author(s): Matsumoto Y
  • Organizer: 12th Taiwan-Japan Conference on Otolaryngology Head and Neck Surgery
  • Place of Presentation: National Taiwan University Hospital, Taipei City, Taiwan
  • Year and Date: 2013-12-06
• [Presentation] Intravital Calcium Imaging of Cochlear Hair Cells Upon Sound Stimulation (2013)
  • Author(s): Matsumoto Y
  • Organizer: 50th Inner Ear Biology Workshop
  • Place of Presentation: Universidad de Alcalá, Alcalá de Henares, Madrid, Spain
  • Year and Date: 2013-09-11
• [Presentation] Intravital Calcium Imaging of Cochlear Hair Cells (2013)
  • Author(s): Matsumoto Y
  • Organizer: 36th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: Baltimore Marriott Waterfront Hotel, Baltimore, MD, USA
  • Year and Date: 2013-02-18
• [Presentation] Intravital Confocal Microscopy for Dds Evaluation and Development (2013)
  • Author(s): Matsumoto Y, Sheraton
  • Organizer: 16th International Symposium on Recent Advances in Drug Delivery Systems
  • Place of Presentation: Salt Lake City Hotel, Salt Lake City, UT, USA
  • Year and Date: 2013-02-06
• [Presentation] 生体内リアルタイム共焦点顕微鏡の手技の実際 (2012)
  • Author(s): 松本有
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、北海道札幌市
  • Year and Date: 2012-07-05
• [Presentation] 生体内リアルタイム共焦点顕微鏡によるDDS挙動解析 (2012)
  • Author(s): 松本有
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、北海道札幌市
  • Year and Date: 2012-07-05
• [Presentation] Imaging DDS Using Intravital Real-Time Confocal Microscopy (2011)
  • Author(s): Matsumoto Y
  • Organizer: The 11th US-Japan Symposium on Drug Delivery Systems
  • Place of Presentation: The Westin Maui Resort and Spa, Lahaina, Maui, HI, USA
  • Year and Date: 2011-12-18
• [Presentation] 生体内リアルタイム共焦点顕微鏡と蝸牛イメージングへの展開 (2011)
  • Author(s): 松本有
  • Organizer: 第21回日本耳科学会総会
  • Place of Presentation: 学術講演会,沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2011-11-24
• [Presentation] 生体内リアルタイム共焦点顕微鏡を用いたDDS機能評価 (2011)
  • Author(s): 松本有
  • Organizer: 第27回日本DDS学会
  • Place of Presentation: 東京大学、東京都文京区
  • Year and Date: 2011-06-09
• [Presentation] Intravital Confocal Micro - Videography (2011)
  • Author(s): Matsumoto Y
  • Organizer: International Conference on Biomaterials Science (ICBS) 2011
  • Place of Presentation: Tsukuba International Congress Center, Tsukuba, Ibaraki, Japan
  • Year and Date: 2011-03-16
• [Book] Intravital Real-Time Confocal Laser Scanning Microscopy for the in Situ Evaluation of Nanocarriers (2013)
  • Author(s): Matsumoto Y, Nomoto T, Toh K, Cabral H, Murakami M, Christie RJ, Kim HJ, Ogura T, Miyata K, Nishiyama N, Yamasoba T, Kataoka K, Cancer Targeted Drug Delivery, Bae, Y. H., Mrsny, R. J., and Park, K.s. Bae, Y. H., Mrsny, R. J., and Park, K
  • Total Pages: 607-620
  • Publisher: Springer New York
• [Remarks]
  • URL: http://www.bmw.t.u-tokyo.ac.jp/
• [Patent(Industrial Property Rights)] 薬剤送達用のキャリア、コンジュゲートおよびこれらを含んでなる組成物並びに投与方法 (2013)
  • Inventor(s): 片岡一則,安楽泰孝,西山伸宏,宮田 完二郎,石井武彦,松本有,福里優,溝口明祐,横田隆徳,桑原宏哉,仁科一隆,水澤英洋
  • Industrial Property Rights Holder: 東京大学工学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学医学系研究科,東京大学工学系研究科,東京大学医学部附属病院,東京大学工学系研究科,東京大学医学系研究科,国立大学法人東京医科歯科大学,国立大学法人東京医科歯科大学,国立大学法人東京医科歯科大学,国立大学法人東京医科歯科大学
  • Industrial Property Number: 特願2013-242347
  • Filing Date: 2013-11-22
• [Patent(Industrial Property Rights)] 粒子組成物および該粒子組成物を用いた医薬組成物 (2012)
  • Inventor(s): 片岡一則,ロナルドジェイムズクリスティ,西山伸宏,宮田完二郎,福島重人,松本有,野本貴大,加藤泰己
  • Industrial Property Rights Holder: 東京大学工学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学医学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学工学系研究科,ナノキャリア株式会社
  • Industrial Property Number: 12733905.9
  • Filing Date: 2012-01-16
  • Overseas
• [Patent(Industrial Property Rights)] 粒子組成物および該粒子組成物を用いた医薬組成物 (2012)
  • Inventor(s): 片岡一則,ロナルドジェイムズクリスティ,西山伸宏,宮田完二郎,福島重人,松本有,野本貴大,加藤泰己
  • Industrial Property Rights Holder: 東京大学工学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学医学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学工学系研究科,ナノキャリア株式会社
  • Industrial Property Number: 特願2011-006318
  • Filing Date: 2012-01-14
• [Patent(Industrial Property Rights)] 粒子組成物および該粒子組成物を用いた医薬組成物 (2012)
  • Inventor(s): 片岡一則,ロナルドジェイムズクリスティ,西山伸宏,宮田完二郎,福島重人,松本有,野本貴大,加藤泰己
  • Industrial Property Rights Holder: 東京大学工学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学医学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学工学系研究科,ナノキャリア株式会社
  • Industrial Property Number: 特願2012-552778
  • Filing Date: 2012-01-16
• [Patent(Industrial Property Rights)] 粒子組成物および該粒子組成物を用いた医薬組成物 (2012)
  • Inventor(s): 片岡一則,ロナルドジェイムズクリスティ,西山伸宏,宮田完二郎,福島重人,松本有,野本貴大,加藤泰己
  • Industrial Property Rights Holder: 東京大学工学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学医学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学工学系研究科,ナノキャリア株式会社
  • Industrial Property Number: 13/979546
  • Filing Date: 2012-01-16
  • Overseas
• [Patent(Industrial Property Rights)] 名称:粒子組成物および該粒子組成物を用いた医薬組成物 (2012)
  • Inventor(s): 片岡一則,ロナルドジェイムズクリスティ,西山伸宏,宮田完二郎,福島重人,松本有,野本貴大,加藤泰己
  • Industrial Property Rights Holder: 東京大学工学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学医学系研究科,東京大学工学系研究科,東京大学医学系研究科,東京大学工学系研究科,ナノキャリア株式会社
  • Industrial Property Number: PCT/JP2012/050683
  • Filing Date: 2012-01-16
  • Overseas
• [Patent(Industrial Property Rights)] 複合体微粒子およびその製造方法、ならびに該複合体微粒子を用いた薬学組成物 (2011)
  • Inventor(s): 片岡一則,宮田完二郎,西山伸宏,大 庭誠,山崎裕一,須磨知也,グダノハ,武元宏泰,松本有,野本貴大
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 特願2011-552789
  • Filing Date: 2011-02-02
• [Patent(Industrial Property Rights)] 複合体微粒子およびその製造方法、ならびに該複合体微粒子を用いた薬学組成物 (2011)
  • Inventor(s): 片岡一則,宮田完二郎,西山伸宏,大 庭誠,山崎裕一,須磨知也,グダノハ,武元宏泰,松本有,野本貴大
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: PCT/JP2011/052081
  • Filing Date: 2011-02-02
  • Overseas