2012 Fiscal Year Final Research Report
Development of functional mitochondrial imaging using electron mediator
| Project/Area Number |
23790050
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
HYODO Fuminori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (10380693)
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| Project Period (FY) |
2011 – 2012
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| Keywords | イメージング / レドックス / ミトコンドリア / 電子伝達体 |
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| Research Abstract |
Regulationof tissue redox status is an important to maintain normal physiological conditions in the living body. Breakdown of redox homoeostasis may lead to oxidative stress and can induce many pathological conditions such as neurological disorders, inflammation, cancer, and ageing. Our energy production system in mitochondria produces ATP during various redox reaction using endogenous molecules such as ubiquinone (CoQ) and FAD. Overhauser-MRI (OMRI) enables visualization of tissue redox status in animals based on dynamic nuclear polarization (DNP) using radical probes such as nitroxyl radicals and trityl radical non-invasively. We have developed home-made OMRI systems and more recently constructed high field OMRI system using circularly transporting system of a sample for high-sensitive and redox analysis based on anatomical structure by MRI. In this report, we demonstrate capability of visualization of ubiquinone using OMRI systems for monitoring mitochondrial function non-invasively.
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