Development of a novel fluorescence derivatization method for the screening of the risk of side effects caused by anticancer drugs

2014 Fiscal Year Final Research Report

• Project/Area Number: 23790051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: SHIBATA Takayuki 長崎大学, 医歯薬学総合研究科(薬学系), 助教 (10448491)
• Research Collaborator: FUJITA Jun-ya ; DRAGUSHA Shpend ; YIN Sheng ; SHIMAMURA Ryosuke
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: DPD欠損症 / ウラシル / ベンズアミドオキシム / 蛍光反応 / 臨床検査 / スクリーニング / シトシン / オロト酸

Outline of Final Research Achievements

In this research, a uracil-specific fluorogenic reaction developed by our research group was applied to the quantification of urinary uracil concentration for the development of a facile and rapid diagnostic method of DPD deficiency. As a result, we have succeeded in a simultaneous analysis of urinary uracil level using multiple samples, which requires a simple addition of aqueous reagent solutions and heating for a few minutes. The result suggested that the present method would be applicable to the screening test of DPD deficiency at a clinical site. In addition, by changing the reaction conditions of the uracil-specific fluorogenic reaction, we also have discovered novel fluorescence derivatization methods specific to cytosine and orotic acid, respectively.

Free Research Field

有機化学、分析化学