Structure determination and elucidation of transport mechanism of mitochondrial inner membrane transporter

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790064
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAKEUCHI Koh 独立行政法人産業技術総合研究所, 創薬分子プロファイリング研究センター, 主任研究員 (20581284)
• Project Period (FY): 2011 – 2012
• Keywords: 構造生物学

Research Abstract

To contribute the understanding and cure of the human diseases associated with mitochondrial membrane transporters (MTPs), we developed prokaryotic expression systems for 13 types of MTPs for structure determination and elucidation of their transport mech

Research Products

• [Journal Article] Functional dynamics of proteins revealed by solution NMR. (2012)
  • Author(s): Osawa M, Takeuchi K, Ueda T, Nishida N, Shimada I
  • Journal Title: Curr Opin Struct Biol Volume: 22 Pages: 660-669
  • DOI: Doi:10.1016/j.sbi.2012.08.007
  • Peer Reviewed
• [Journal Article] Structural Basis for the Golgi Association by the Pleckstrin Homology Domain of the Ceramide Trafficking Protein (CERT) (2012)
  • Author(s): Sugiki T, Takeuchi K, Yamaji T, Takano Y, Tokunaga Y, Kumagai K, Hanada K, Takahashi H, Shimada I
  • Journal Title: J Biol Chem Volume: 287 Pages: 33706-33718
  • DOI: Doi:10.1074/jbc.M112.367730
  • Peer Reviewed
• [Journal Article] Speeding up direct (15)N detection: hCaN 2D NMR experiment (2011)
  • Author(s): Gal M, Edmonds KA, Milbradt AG, Takeuchi K, Wagner G
  • Journal Title: J Biomol NMR Volume: 51 Pages: 497-504
  • DOI: DOI:10.1007/s10858-011-9580-7
  • Peer Reviewed
• [Journal Article] Structure of the VP16 transactivator target in the Mediator (2011)
  • Author(s): Milbradt AG, Kulkarni M, Yi T, Takeuchi K, Sun ZY, Luna RE, Selenko P, Naar AM, Wagner G
  • Journal Title: Nat Struct Mol Biol Volume: 18 Pages: 410-418
  • DOI: Doi:10.1038/nsmb.1999
  • Peer Reviewed
• [Presentation] 多剤耐性転写制御因子の揺らぎによる薬剤認識機構 (2012)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 新学術領域「揺らぎと生体機構」「水和と ATP」合同公開シンポジウム「ゆらぎと水-生命のエネルギーと機能の分子機構を探る」
  • Place of Presentation: 大阪ガーデンパレス(大阪府)
  • Year and Date: 2012-09-14
• [Presentation] NMR approach to study and interfere with blood coagulation responses (2012)
  • Author(s): 竹内 恒
  • Organizer: 難治性疾患統合創薬プロジェクト講演会
  • Place of Presentation: 立命館大学(滋賀県)
  • Year and Date: 2012-01-30
• [Presentation] HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment (2011)
  • Author(s): Koh Takeuchi, Maayan Gal, Hideo Takahashi, Gerhard Wagner, and Ichio Shimada
  • Organizer: ISNMR2011
  • Place of Presentation: 大桟橋ホール(神奈川県)
  • Year and Date: 20111115-18
• [Presentation] T 細胞レセプターはダイナミックかつ協同的な 4 次元構造変化により活性化する-NMR および光ピンセット法による解析- (2011)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路工大(兵庫県)
  • Year and Date: 20110916-18
• [Book] Royal Society of Chemistry,Recent Developments in Biomolecular NMR (2012)
  • Author(s): Takeuchi K, Gal M, Shimada I, and Wagner G 他
  • Total Pages: 364
• [Remarks]
  • URL: http://unit.aist.go.jp/birc2/