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2012 Fiscal Year Final Research Report

Establishment of the analytical method of phosphoinositide by using tandem mass spectrometry.

Research Project

  • PDF
Project/Area Number 23790074
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionAkita University

Principal Investigator

NAKANISHI Hiroki  秋田大学, 生体情報研究センター, 助教 (10466740)

Project Period (FY) 2011 – 2012
Keywordsリピドミクス / ホスホイノシタイド / 高感度分析法
Research Abstract

We developed a sensitive and robust method to determine PIPs with different fatty acid compositions by using tandem mass spectrometry. This established analytical method was applicable to various biological samples such as cells and mouse tissues. In this research, we found that fatty acid compositions of PIPs had diversity.

  • Research Products

    (17 results)

All 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (4 results) Book (3 results) Remarks (1 results)

  • [Journal Article] 酸性リゾリン脂質を量る2013

    • Author(s)
      中西広樹
    • Journal Title

      実験医学別冊

      Pages: 118-123

  • [Journal Article] The Lipid Mediator Protectin D1 Inhibits Influenza Virus Replication and Improves Severe Influenza2013

    • Author(s)
      Morita M., Nakanishi H., Imai Y.
    • Journal Title

      Cell

      Volume: 153 Pages: 1-14

    • DOI

      DOI:10.1016/j.cell.2013.02.027.

    • Peer Reviewed
  • [Journal Article] Global metabolomic analysis of heart tissue in a hamster model for dilated cardiomyopathy2013

    • Author(s)
      Maekawa K., Nakanishi H., Saito Y.
    • Journal Title

      J. Mol. Cell Cardiol

      Volume: 59C Pages: 76-85

    • DOI

      DOI:10.1016/j.yjmcc.2013.02.008.

    • Peer Reviewed
  • [Journal Article] Novel regulation of cardiac metabolism and homeostasis by the adrenomedullin-receptor activity-modifying protein 2 system2013

    • Author(s)
      Yoshizawa T., Nakanishi H., Shindo T.
    • Journal Title

      Hypertension

      Volume: 61 Pages: 341-351

    • DOI

      DOI:10.1161/HYPERTENSIONAHA.111.006 47.

    • Peer Reviewed
  • [Journal Article] Identification and Structure Determination of Novel Anti-inflammatory Mediator Resolvin E3, 17,18-Dihydroxyeicosapentaenoic Acid2012

    • Author(s)
      Isobe Y., Arita M., Nakanishi H., Arai H.
    • Journal Title

      J. Biol. Chem

      Volume: 287 Pages: 10525-10534

    • DOI

      DOI:10.1074/jbc.M112.340612

    • Peer Reviewed
  • [Journal Article] LYCAT, a homologue of C. elegans acl-8, acl-9, and acl-10, determines the fatty acid composition of phosphatidylinositol in mice2012

    • Author(s)
      Imae R., Nakanishi H., Arai H.
    • Journal Title

      J. Lipid Res

      Volume: 53 Pages: 335-347

    • DOI

      DOI:10.1194/jlr.M018655

    • Peer Reviewed
  • [Journal Article] 酸性リン脂質の高感度分析法とその臨床的有意性2011

    • Author(s)
      中西広樹
    • Journal Title

      JSB MS Letters・日本医用マススペクトル学会誌

      Volume: 36 Pages: 24-30

  • [Journal Article] p125/Sec23-interacting protein (Sec23ip) is required for spermiogenesis2011

    • Author(s)
      Arimitsu N., Nakanishi H., Tani K.
    • Journal Title

      FEBS Lett

      Volume: 585 Pages: 2171-2176

    • DOI

      DOI:10.1016/j.febslet.2011.05.05 0

    • Peer Reviewed
  • [Journal Article] Hair follicular expression and function of group X secreted phospholipase A2 in mouse skin2011

    • Author(s)
      Yamamoto K., Nakanishi H., Murakami M.
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 11616-11631

    • DOI

      DOI:10.1074/jbc.M110.206714

    • Peer Reviewed
  • [Presentation] 質量分析計を用いたイノシトールリン脂質の一斉定量分析系の開発2012

    • Author(s)
      中西広樹
    • Organizer
      第54回日本脂質生化学会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2012-06-07
  • [Presentation] 脂質メタボロミクスから理解する炎症性疾患2011

    • Author(s)
      中西広樹
    • Organizer
      第48回シグナル伝達医学グローバルCOE 学術講演会
    • Place of Presentation
      神戸大学(神戸市)
    • Year and Date
      2011-09-07
  • [Presentation] Analyses of lipid localization/lipid metabolism on mouse retina using laser microdissection2011

    • Author(s)
      Hiroki Nakanishi
    • Organizer
      The 30^<th> Naito Conference
    • Place of Presentation
      シャトレーゼ ガトーキングダム サッポロ(札幌市)
    • Year and Date
      2011-06-29
  • [Presentation] 質量分析計を用いたイノシトールリン脂質の高感度測定2011

    • Author(s)
      中西広樹
    • Organizer
      第10回生命科学研究会
    • Place of Presentation
      ホテルメトロポリタン高崎(高崎市)
    • Year and Date
      2011-06-24
  • [Book] Lipidomics, Technologies and Applications2012

    • Author(s)
      Taguchi R., Ikeda K., Nakanishi H
    • Total Pages
      223-250
    • Publisher
      Lipidomics for elucidation of Metabolic Syndrome and related Lipid Metabolic Disorder
  • [Book] 試料分析講座・脂質分析2011

    • Author(s)
      中西広樹
    • Total Pages
      101-118
    • Publisher
      リン脂質およびリン脂質酸化物の分析
  • [Book] 試料分析講座・脂質分析2011

    • Author(s)
      中西広樹
    • Total Pages
      171-177
    • Publisher
      MS イメージングによる脂質の局在解析
  • [Remarks]

    • URL

      http://www.gipc.akita-u.ac.jp/~arcbs/in dex.html

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Published: 2014-09-25  

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