The analysis of cellular osmotic stress sensing and response by focusing onASK3

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790078
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: NAGURO Isao 東京大学, 大学院・薬学系研究科, 講師 (80401222)
• Project Period (FY): 2011 – 2012
• Keywords: ストレス応答 / RNAi スクリーニング / 浸透圧ストレス / ASK3

Research Abstract

In our project, we focused on an osmotic stress-responsive kinase ASK3 to investigate how organisms sense and respond to the stress. We preformed a screening for the regulator of ASK3 upon osmotic stress, and found several proteins that are involved in the osmotic stress response in the cells. Furthermore, we identified that ASK3 is involved in the control of systemic blood pressure.

Research Products

• [Journal Article] Activation mechanisms of ASK1 in responseto various stresses and its significance inintracellular signaling. (2013)
  • Author(s): Shiizaki, S., Naguro, I. and Ichijo, H.
  • Journal Title: Adv. Biol. Regul., (review article) Volume: 53 Pages: 135-144
  • DOI: doi:10.1016/j.jbior.2012.09.006
  • Peer Reviewed
• [Journal Article] A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants. (2012)
  • Author(s): Fujisawa, T., Homma, K., Yamaguchi, N., Kadowaki, H., Tsuburaya, N., Naguro, I., Matsuzawa, A., Takeda, K., Takahashi, Y., Goto, J., Tsuji, S., Nishitoh, H. and Ichijo, H.
  • Journal Title: Ann. Neurol. Volume: 72 Pages: 739-749
  • DOI: doi:10.1002/ana.23668.
  • Peer Reviewed
• [Journal Article] ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney. (2012)
  • Author(s): Naguro, I., Umeda, T., Kobayashi, Y., Maruyama, J., Hattori, K., Shimizu, Y., Kataoka, K., Kim-Mitsuyama, S., Uchida, S., Vandewalle, A., Noguchi, T., Nishitoh, H., Matsuzawa, A., Takeda, K. and IchijoH.
  • Journal Title: Nat. Commun. Volume: 3 Pages: 1285
  • DOI: doi:10.1038/ncomms2283
  • Peer Reviewed
• [Journal Article] p38MAP kinase regulates the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors. (2011)
  • Author(s): Sekine,Y., Takagahara,S., Hatanaka, R., Watanabe, T., Oguchi, H., Noguchi, T., Naguro, I., Kobayashi, K., Tsunoda, M., Funatsu, T., Nomura, H., Toyoda, T., Matsuki, N., Kuranaga, E., Miura, M., Takeda, K., and Ichijo, H.
  • Journal Title: J. Cell Sci. Volume: 124 Pages: 3006-3016
  • DOI: doi:10.1242/jcs.085902
  • Peer Reviewed
• [Presentation] 浸透圧応答性キナーゼ ASK3のHaloTagテクノロジーを利用した 結合分子探索 (2013)
  • Author(s): 名黒 功、一條 秀憲
  • Organizer: 第7回メカノセンシング研究会
  • Place of Presentation: 札幌
  • Year and Date: 2013-03-07
• [Presentation] 浸透圧ストレスに両方向性に応答するASK3によるWNK1-SPAK/OSR1経路の制御 (2012)
  • Author(s): 名黒 功, 一條 秀憲
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121214-1216
• [Presentation] The role of an osmoticstress-responsive kinase ASK3 and anapproach to the molecular mechanism of thecellular osmo-sensing (2012)
  • Author(s): Naguro, I., Ichijo, H.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜.
  • Year and Date: 20121213-1216
• [Presentation] ASK3の浸透圧ストレス応答とWNK1-SPAK/OSR1経路の制御 (2011)
  • Author(s): 名黒功、一條秀憲
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110921-0945
• [Presentation] 浸透圧ストレス応答のシグナリング (2011)
  • Author(s): 名黒功、一條秀憲
  • Organizer: 第3回シグナルネットワーク研究会
  • Place of Presentation: 東京
  • Year and Date: 20110527-0528