A novel cholinergic enhancer and its mechanism of action

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790097
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Keio University
• Principal Investigator: OKUDA Takashi 慶應義塾大学, 薬学部, 講師 (00322040)
• Project Period (FY): 2011 – 2012
• Keywords: コリン作動性神経 / アセチルコリン / コリントランスポーター / トラフィッキング / トポロジー / オリゴマー

Research Abstract

The high-affinity choline transporter (CHT1) is responsible for choline uptake,the rate-limiting step in acetylcholine synthesis at the cholinergic presynaptic terminals. Little is known about the regulation of the CHT1 trafficking or the structure-func

Research Products

• [Journal Article] Transmembrane topology and oligomeric structure of the high-affinity choline transporter (2012)
  • Author(s): Okuda, T., Osawa, C., Yamada, H., Hayashi, K., Nishikawa, S., Ushio, T., Kubo, Y., Satou, M., Ogawa, H., and Haga, T
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 42826-42834
  • Peer Reviewed
• [Journal Article] Osteopontin is an alpha motor neuron marker in the mouse spinal cord. (2012)
  • Author(s): Misawa, H., Hara, M., Tanabe S., Niikura, M., Moriwaki, Y., and Okuda, T
  • Journal Title: J. Neurosci. Res Volume: 90 Pages: 732-742
  • Peer Reviewed
• [Journal Article] Substrate-induced internalization of the high-affinity choline transporter (2011)
  • Author(s): Okuda, T., Konishi, A., Misawa, H., and Haga, T
  • Journal Title: J. Neurosci. Volume: 87 Pages: 3024-3032
  • Peer Reviewed
• [Presentation] Substrate-induced internalization A novel screen for endocytic motifs of vesicular neurotransmitter transporters (2012)
  • Author(s): 小山沙織、美濃田茜、神保美紗子、三澤日出巳、奥田隆志
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] シナプス小胞トランスポーターの細胞内移行モチーフの探 (2011)
  • Author(s): 神保美紗子、三澤日出巳、奥田隆志
  • Organizer: 第124回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-04
• [Book] コリン作動性神経機能素子の制御機構、基礎老化研究36 (3) (2012)
  • Author(s): 奥田隆志
  • Total Pages: 25-29
• [Book] 高親和性コリントランスポーターの局在と機能、Clinical Neuroscience 30 (2012)
  • Author(s): 奥田隆志
  • Total Pages: 640-641
• [Book] 神経変性疾患と創薬、新しい薬学事典 (2012)
  • Author(s): 奥田隆志
  • Total Pages: 95-97
  • Publisher: 朝倉書店