Identification of alternative pentose phosphate pathway in animals.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790122
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: KONDO Yoshitaka 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (20507397)
• Project Period (FY): 2011 – 2013
• Keywords: 代謝 / 生化学 / 酵素 / 動物 / ペントースリン酸経路 / グルコン酸キナーゼ

Research Abstract

In pentose phosphate pathway, glucose 6-phosphate is converted to glyceraldehyde 3-phosphate via glucono-1,5-lactone 6-phosphate and 6-phospho gluconate to produce NADPH and various hexose which is necessary for a synthesis of nucleic acid. It is known that alternative pathway from glucose to 6-phospho gluconate through gluconolactone and gluconate exists in bacteria. However, it remains unclear whether the alternative pathway exists in animals. In this study, we identified the gluconokinase gene which catalyzes a phosphorylation of gluconate to 6-phospho gluconate in human and mouse.

Research Products

• [Journal Article] Senescence marker protein-30/Superoxide dismutase 1 double knockout mice exhibits increased oxidative stress and hepatic steatosis (2014)
  • Author(s): Kondo, Y., Masutomi, H., Noda, Y., Ozawa, Y., Takahashi, T., Handa, S., Maruyama, N., Shimizu, T., and Ishigami, A
  • Journal Title: FEBS Open Bio Volume: 4 Pages: 522-532
  • DOI: 10.1016/j.fob.2014.05.003
  • Peer Reviewed
• [Journal Article] Senescence marker protein-30 (SMP30)/ gluconolactonase (GNL) expression in the mouse ovary during gestation (2013)
  • Author(s): Kagami, Y., Kondo, Y., Handa, S., Maruyama, N., and Ishigami, A
  • Journal Title: Biol. Pharm. Bull Volume: 36(12) Pages: 2005-2008
  • DOI: 10.1248/bpb.b13-00309
  • Peer Reviewed
• [Journal Article] Lepr^<db/db> mice with senescence marker protein-30 knockout (Lepr^<db/db> Smp30 ^<Y/->) exhibit increases in small dense-LDL and severe fatty liver despite being fed a standard diet (2013)
  • Author(s): Kondo, Y., Hasegawa, G., Okada, H., Senmaru, T., Fukui, M., Nakamura, N., Sawada, M., Kitawaki, J., Okanoue, T., Kishimoto, Y., Amano, A., Maruyama, N., Obayashi, H., and Ishigami, A
  • Journal Title: PLoS ONE Volume: 8(6) Pages: 65698
  • DOI: 10.1371/journal.pone.0065698
  • Peer Reviewed
• [Presentation] SMP30を欠損したLepr^<db/db>マウスはsmall dense LDLが高く脂肪肝を呈する (2013)
  • Author(s): 近藤嘉高、長谷川剛二、千丸貴史、福井道明、中村直登、丸山直記、尾林博、石神昭人
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] ペントースリン酸副経路で働くヒトおよびマウスにおけるグルコン酸キナーゼの同定 (2013)
  • Author(s): 近藤嘉高、半田節子、丸山直記、石神昭人
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-13
• [Presentation] Smp30/Sod1ダブルノックアウトマウスにおける脂質代謝異常の解析 (2012)
  • Author(s): 近藤嘉高、半田節子、野田義博、丸山直記、清水孝彦、石神昭人
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Remarks] 研究室ホームページ
  • URL: http://www.aging-regulation.jp/
• [Remarks] 東京都健康長寿医療センター研究所 分子老化制御
  • URL: http://www.tmghig.jp/J_TMIG/kenkyu/team/bunshiroukaseigyo.html