Association between siderophores produced by pathogenic vibrios and host inflammatory response

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental pharmacy
• Research Institution: Matsuyama University
• Principal Investigator: TANABE Tomotaka 松山大学, 薬学部, 助教 (60532786)
• Project Period (FY): 2011 – 2012
• Keywords: シデロフォア / 鉄 / 病原ビブリオ / 腸炎ビブリオ

Research Abstract

Iron is an essential element for bacterial survival, and its acquisition is crucial for the ability of most pathogenic bacteria to establish and maintain an infection within the host. Vibrio parahaemolyticus, a human pathogen causing watery diarrhea, can produce vibrioferrin, a type of siderophore which is a low-molecular-weight iron-binding chelator produced by microbes, and can acquire iron provided by iron-vibrioferrin complex. In this study, I clarified a novel regulatory system for production of vibrioferrin. Moreover, I examined a physiological function of vibrioferrin against host cells, and also assessed the influence of the deletion of gene involved in vibrioferrin production on cytotoxicity of V. parahaemolyticus.

Research Products

• [Journal Article] Characterization of Vibrio parahaemolyticus genes encoding the systems for utilization of enterobactin as a xenosiderophore. (2012)
  • Author(s): Tanabe, T., Funahashi, T., Shiuchi, K., Okajima, N., Nakao, H., Miyamoto, K., Tsujibo, H., and Yamamoto, S.
  • Journal Title: Microbiology-SGM Volume: 158 Pages: 2039-2049
  • Peer Reviewed
• [Journal Article] The Vibrio parahaemolyticus pvuA1 gene(formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2. (2011)
  • Author(s): Tanabe, T., Funahashi, T., Okajima, N., Nakao, H., Takeuchi, Y., Miyamoto, K., Tsujibo, H., and Yamamoto, S.
  • Journal Title: FEMS Microbiol Lett Volume: 324 Pages: 73-79
  • Peer Reviewed
• [Journal Article] Identification of genes, desR and desA, required for utilization of desferrioxamine B as a xenosiderophore in Vibrio furnissii. (2011)
  • Author(s): Tanabe, T., Funahashi, T., Miyamoto, K., Tsujibo, H., and Yamamoto, S.
  • Journal Title: Biol Pharm Bull Volume: 34 Pages: 570-574
  • Peer Reviewed
• [Journal Article] The Vibrio parahaemolyticus small RNA RyhB promotes production of the siderophore vibrioferrin by stabilizing the polycistronic mRNA.
  • Author(s): Tanabe, T., Funahashi, T., Nakao, H., Maki, J., and Yamamoto, S.
  • Journal Title: J Bacteriol Volume: (in press)
  • Peer Reviewed
• [Presentation] The vibrioferrin biosynthesis operon in Vibrio parahaemolyticus is upregulated by the small RNA RyhB (2013)
  • Author(s): 田邊 知孝、舟橋達也、山本重雄
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] 腸炎ビブリオにおける小分子RNA RyhB と RNA結合蛋白質 Hfq が関与する vibrioferrin 生合成オペロン mRNA の安定化 (2012)
  • Author(s): 田邊知孝、舟橋 達也、山本重雄
  • Organizer: 第46回腸炎ビブリオシンポジウム
  • Place of Presentation: 由布市
  • Year and Date: 20121115-16
• [Presentation] 腸炎ビブリオのvibrioferrin生合成オペロンmRNAはHfqを介するsmallRNARyhBの結合により安定化する (2012)
  • Author(s): 田邊知孝、舟橋達也、山本重雄
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島市
  • Year and Date: 20121020-21
• [Presentation] 腸炎ビブリオのsmall RNA RyhBによるvibrioferrin生合成遺伝子の発現制御 (2012)
  • Author(s): 田邊知孝、舟橋達也、山本重雄
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌市
  • Year and Date: 20120328-31
• [Presentation] 腸炎ビブリオのsmall RNA RyhBによるvibrioferrin産生調節機構の解析 (2011)
  • Author(s): 田邊知孝、舟橋達也、山本重雄
  • Organizer: 第64回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山市
  • Year and Date: 20111022-23
• [Presentation] 腸炎ビブリオのvibrioferrin産生におけるsmall RNA RyhBの関与 (2011)
  • Author(s): 田邊知孝、舟橋達也、山本重雄
  • Organizer: 第45回腸炎ビブリオシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20111020-21
• [Presentation] Utilization of xenosiderophores by Vibrioparahaemolyticus: Identification and characterization of genes, irgA, vctA and VPA0150, encoding ferric enterobactin receptors (2011)
  • Author(s): Tanabe, T., Funahashi, T., Miyamoto, K., Tsujibo, H., and Yamamoto, S.
  • Organizer: International Union of MicrobiologicalSocieties2011
  • Place of Presentation: 札幌市
  • Year and Date: 20110906-10
• [Presentation] The Vibrio parahaemolyticus psuA gene encodes a second ferric vibrioferrin receptor exclusively dependent on the TonB2 system.nternational (2011)
  • Author(s): Tanabe, T., Funahashi, T., Nakao, H., Miyamoto, K., Tsujibo, H., and Yamamoto, S.
  • Organizer: Union of Microbiological Societies2011
  • Place of Presentation: 札幌市
  • Year and Date: 20110906-10