2012 Fiscal Year Final Research Report
Regulation of alternative splicing during neural development
| Project/Area Number |
23790218
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経発生 / RNA splicing / 発生工学 |
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| Research Abstract |
The functional coupling roles of alternative splicing and nonsense mediated mRNA decay in the developing brain had remained unclear. Therefore we generated conditional KO mouse of splicing regulatory factor PTB. We found that a loss of PTB caused serious hydrocephalus. PTB was expressed in neural stem cells and required for the maintenance of the adhesion structure of Radial glia formed in the ventricular zone, and the undifferentiated state.Furthermore, we generated UPF1 conditional KO mouse and found that UPF1 is essential for cell proliferation and neuronal differentiation.
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