2013 Fiscal Year Final Research Report
Analysis of the regulatory mechanism and function of stathmin1 in the central nervous system
| Project/Area Number |
23790227
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hamamatsu University School of Medicine (2013)
Osaka University (2011-2012) |
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Principal Investigator |
YAMADA Kohei 浜松医科大学, 子どものこころの発達研究センター, 講師 (50588879)
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| Project Period (FY) |
2011 – 2013
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| Keywords | stathmin1 / 神経 / リン酸化タンパク質 / 脳発達 / 周産期 |
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| Research Abstract |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide involved in a variety of brain function. We demonstrated that PACAP regulates stathmin1 expression. Shumyatsky GP. et al. reported that mice without stathmin1 have deficiency in innate and learned fear. However, the detailed function and regulatory mechanism of stathmin1 in the central nervous system are unclear. In this study, we found that stathmin1 was phosphorylated during perinatal stage in the cortex. Phosphorylated stathmin1 was localized in the marginal zone of cortex, forming dot like fibers. In primary cultured neuron study, we detected dot-like phosphorylated stathmin1. And we indicated that BDNF was related to phosphorylation of stathmin1. PC12 cells over-expressed stathmin1 constitutive active form had longer thick neurites and numerous slight neurites as a filopodia. These results show that phosphorylated stathmin1 is related to filopodia or synapse constitution during perinatal period.
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[Journal Article] DISC1 (disrupted-in-schizophrenia-1) regulates differentiation of oligodendrocytes2014
Author(s)
Hattori T, Shimizu S, Koyama Y, Emoto H, Matsumoto Y, Kumamoto N, Yamada K, Takamura H, Matsuzaki S, Katayama T, Tohyama M, Ito A
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Journal Title
PLoS One
Volume: 9(2)
Pages: e88506
DOI
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[Journal Article] XLMR protein related to neurite extension (Xpn/KIAA2022) regulates cell-cell and cell-matrix adhesion and migration2013
Author(s)
Magome T, Hattori T, Taniguchi M, Ishikawa T, Miyata S, Yamada K, Takamura H, Matsuzaki S, Ito A, Tohyama M, Katayama T
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Journal Title
Neurochem Int
Volume: 63(6)
Pages: 561-569
DOI
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[Journal Article] DBZ (DISC1-binding zinc finger protein)-deficient mice display abnormalities in basket cells in the somatosensory cortices2013
Author(s)
Koyama Y, Hattori T, Shimizu S, Taniguchi M, Yamada K, Takamura H, Kumamoto N, Matsuzaki S, Ito A, Katayama T, Tohyama M
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Journal Title
J Chem Neuroanat
Volume: 53
Pages: 1-10
DOI
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