2012 Fiscal Year Final Research Report
Investigation of the mechanism of ion permeation through a highly cytotoxic peptide channel
| Project/Area Number |
23790245
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生体膜 / チャネル / ペプチド / 細胞毒性 / 生理活性 |
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| Research Abstract |
In this study ion permeation mechanism of a highly cytotoxicpeptide from a marine sponge, polytheonamide B (pTB), was evaluated by use of planer lipid bilayer. We found that the pTB channel had one-ion pore. This result indicates that the mechanism underlying the ion permeation is different from that of the gramicidin A (gA) channel although both channels have similar β -helical structure. In addition, we revealed that the pTB channel exhibited voltage-dependent gating and fast transition between open and closed state. We suggest that, different from gA channels, the changes in the intra-peptide structure govern the gating of the pTB channel.
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