2013 Fiscal Year Final Research Report
Multifaceted function analyses of fever-developing neurons using genetically modified rats
| Project/Area Number |
23790271
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKAMURA Yoshiko 京都大学, 生命科学系キャリアパス形成ユニット, 研究員 (60548543)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 温熱生理学 / 生体恒常性 / プロスタグランジン / 発熱 / 視索前野 / 遺伝子改変動物 / 光遺伝学 |
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| Research Abstract |
To reveal the core brain mechanism of fever development, I have performed multifaceted analyses of prostaglandin EP3 receptor-expressing neurons in the preoptic area (EP3 neurons), a center for fever development. By creating a novel transgenic rat and viruses, I established a system that allows us to specifically transduce EP3 neurons with a reporter gene. Using this system, I transduced EP3 neurons with a membrane-targeted form of GFP to visualize their axon fibers, and thereby, successfully identified all the brain regions where EP3 neurons project their axons. Also, by illuminating EP3 neurons transduced with channelrhodopsin, I was able to change thermogenic activity of brown adipose tissue in anesthetized rats. I am currently doing electrophysiological analyses of EP3 neurons in brain slices.
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[Journal Article] EP3 receptors mediate PGE2-induced hypothalamic paraventricular nucleus excitation and sympathetic activation2011
Author(s)
Zhang, Z.-H., Yu, Y., Wei, S.-G., Nakamura, Y., Nakamura, K. & Felder, R.B
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Journal Title
Am. J. Physiol
Volume: 301
Pages: H1559-H1569
Peer Reviewed
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