2013 Fiscal Year Final Research Report
Molecular analysis of immune regulation by estrogen signaling through the G-coupled receptor
| Project/Area Number |
23790273
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Azabu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | GPR30 / 自然免疫 / IL-6 / シグナル伝達 / NFkB |
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| Research Abstract |
Estrogen effects the immune response through ERalpha or ERbeta, but recently the involvement of GPR30 (G protein Coupled Receptor 30), a membrane-type estrogen receptor, in anti inflammatory response is reported. This study investigated the molecular mechanism about the regulation of inflammatory cytokines by GPR30. We found that the treatment of GPR30 by G-1, GPR30 specific agonist, suppressed the expression of inflammatory cytokine IL-6 in macrophage cell line stimulated with LPS. G-1 also decreased NFkB promoter activation by LPS, and phosphorylation of kinases which involved in the translocation of transcription factor NFkB from cytosol to nucleus.
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