Elucidation of physiological role of the Bach2 ubiquitination

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790322
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: MUTO Akihiko 東北大学, 大学院・医学系研究科, 准教授 (80343292)
• Project Period (FY): 2011 – 2012
• Keywords: 遺伝子 / 発現制御 / 転写因子 / Bach2 / ユビキチン

Research Abstract

In immune response, B cell fate might be regulated by the amount of ubiquitinated Bach2 proteins. However, in vitro recombinant protein assay revealed that MIPP and Klhl9, which are adaptor proteins in ubiquitination system, didn't enhance the ubiquitination of Bach2 protein. Furthermore, by proteomics analysis using MS spectrometry and mutagenesisfor Bach2 protein, phospholylation sites on Bach2 protein were identified.

Research Products

• [Journal Article] BTB and CNC homolog 1 (Bach1) deficiency ameliorates TNBS colitis in mice: role of M2 macrophages and heme oxygenase-1 (2013)
  • Author(s): Harusato A, Naito Y, Takagi T, Uchiyama K, Mizushima K, Hirai Y, Higashimura Y, Katada K, Handa O, Ishikawa T, Yagi N, Kokura S,Ichikawa H, Muto A, Igarashi K, Yoshikawa T.
  • Journal Title: Inflamm Bowel Dis Volume: 19(4) Pages: 740-753
  • DOI: doi:10.1097/MIB.0b013e3182802968.
  • Peer Reviewed
• [Journal Article] Transcriptional suppression of BACH2 by the Bcr-Abl oncoprotein is mediated by PAX5. (2013)
  • Author(s): Casolari DA, Makri M, Yoshida C, Muto A, Igarashi K, Melo JV.
  • Journal Title: Leukemia Volume: 27(2) Pages: 409-415
  • DOI: doi:10.1038/leu.2012.220.
  • Peer Reviewed
• [Journal Article] Bach1 regulates osteoclastogenesis in a mouse model via both heme oxygenase 1-dependent and heme oxygenase 1-independent pathways (2012)
  • Author(s): Hama M, Kirino Y, Takeno M, Takase K, Miyazaki T, Yoshimi R, Ueda A, Itoh-Nakadai A, Muto A, Igarashi K, Ishigatsubo Y.
  • Journal Title: Arthritis Rheum Volume: 64(5) Pages: 1518-1528
  • DOI: doi:10.1002/art.33497.
  • Peer Reviewed
• [Journal Article] Structure and dynamics of gene regulatory network for humoral immune response (2011)
  • Author(s): Muto A, Itoh-Nakadai A, Igarashi K.
  • Journal Title: Rinsho Ketsueki Volume: 52(6) Pages: 376-386
  • Peer Reviewed
• [Journal Article] Suppression of indomethacin-induced apoptosis in the small intestine due to Bach1 deficiency (2011)
  • Author(s): Harusato A, Naito Y, Takagi T, Uchiyama K, Mizushima K, Hirai Y, Yamada S, Tuji T, Yoriki H, Horie R, Inoue K, Fukumoto K, Handa O, Ishikawa T, Kokura S, Minamiyama Y, Ichikawa H, Muto A, Igarashi K, Yoshikawa T.
  • Journal Title: Free Radic Res Volume: 45(6) Pages: 717-727
  • DOI: doi:10.3109/10715762.2011.574287.
  • Peer Reviewed
• [Journal Article] Heme regulates B-cell differentiation, antibodyclass switch, and heme oxygenase-1 expression in B cells as a ligand of Bach2 (2011)
  • Author(s): Watanabe-Matsui M, Muto A, Matsui T, Itoh-Nakadai A, Nakajima O, Murayama K, Yamamoto M, Ikeda-Saito M, Igarashi K.
  • Journal Title: Blood Volume: 117(20) Pages: 5438-5448
  • DOI: doi:10.1182/blood-2010-07-296483.
  • Peer Reviewed
• [Presentation] B細胞最終分化過程におけるPrdm1遺伝子のエピゲノム制御の解明 (2013)
  • Author(s): 武藤哲彦、田中拡、落合恭子、ブリドンアンドレイ、加藤恭丈、仁尾正記、星川裕、野田哲生、五十嵐和彦
  • Organizer: 若手ワークショップ@鬼怒川
  • Place of Presentation: 鬼怒川
  • Year and Date: 20130124-26
• [Remarks]
  • URL: http://www.biochem.med.tohoku.ac.jp/