Induction of multipotent germline stem cells from germline stem cells by epigenetic change.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790332
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Takashi 京都大学, 医学研究科, 助教 (40579265)
• Project Period (FY): 2011 – 2012
• Keywords: 再生医学

Research Abstract

To el uci date the mechani sm whi ch represses pl uri potency i n germline stem cells (GSCs), this study investigated whether epigenetic changes induce GS-mGS conversion. As a result, lentiviral transduction of shrnas for epigenetic modifiers did not induce GS-mGS conversion. These results suggest that pluripotency in GS cells is repressed by several pathways.

Research Products

• [Journal Article] Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice. (2012)
  • Author(s): Masuda, T., Itoh, K., Higashitsuji, H., Higashitsuji, H., Nakazawa, N., Sakurai, T., Liu, Y., Tokuchi, H., Fujita, T., Zhao, Y., Nishiyama, H., Tanaka, T., Fukumoto, M., Ikawa, M., Okabe, M., Fujita, J.
  • Journal Title: Proceedings of the National Academy Sciences of the United States of America Volume: 109 Pages: 10885-10890
  • DOI: DOI:10.1073/pnas.1121524109
  • Peer Reviewed
• [Journal Article] In vitro transformation of mouse testis cells by oncogene transfection (2012)
  • Author(s): Morimoto, H., Lee, J., Tanaka, T., Ishii, K., Toyokuni, S., Kanatsu-Shinohara, M., Shinohara, T.
  • Journal Title: Biology of Reproduction Volume: 86 Pages: 1-11
  • DOI: DOI:10.1095/biolreprod.111.095307
  • Peer Reviewed
• [Journal Article] Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier (2011)
  • Author(s): Takashima, S., Kanatsu-Shinohara, M., Tanaka, T., Takehashi, M., Morimoto, H., Shinohara, T
  • Journal Title: Cell Stem Cell Volume: 9 Pages: 463-475
  • DOI: DOI:10.1016/j.stem.2011.08.011
  • Peer Reviewed
• [Journal Article] Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis. (2011)
  • Author(s): Tanaka, T., Hosokawa, M., Vagin, VV., Reuter, M., Hayashi, E., Mochizuki, AL., Kitamura, K., Yamanaka, H., Kondoh, G., Okawa, K., Kuramochi-Miyagawa, S., Nakano, T., Sachidanandam, R., Hannon, GJ., Pillai, RS., Nakatsuji, N., Chuma, S.
  • Journal Title: Proceedings of the National Academy Sciences of the United States of America Volume: 108 Pages: 10579-10584
  • DOI: DOI:10.1073/pnas.1015447108
  • Peer Reviewed
• [Journal Article] Homologous recombination in rat germline stem cells. (2011)
  • Author(s): Kanatsu-Shinohara, M., Kato-Itoh, M., Ikawa, M., Takehashi, M., Sanbo, M., Morioka, Y., Tanaka, T., Morimoto, H., Hirabayashi, M., Shinohara, T.
  • Journal Title: Biology of Reproduction Volume: 85 Pages: 208-217
  • DOI: DOI:10.1095/biolreprod.111.090837
  • Peer Reviewed
• [Presentation] インテグリンシグナルによる精子幹細胞制御 (2011)
  • Organizer: 蛋白研セミナー
  • Place of Presentation: 大阪大学蛋白質研究所(招待講演)蛋白質研究所
  • Year and Date: 2011-12-01
• [Remarks]
  • URL: http://www2.mfour.med.kyoto-u.ac.jp/~molgen