The regulatory roles of PAR-aPKC complex in the division patterns of neural stem cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790342
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Yokohama City University
• Principal Investigator: HIROSE Tomonori 横浜市立大学, 医学部, 助教 (20381668)
• Project Period (FY): 2011 – 2012
• Keywords: 神経幹細胞 / 発生・分化 / 細胞極性

Research Abstract

During mammalian cerebral development, neural precursor cells (NPCs) first expand their population and next generate neurons. However, the molecular mechanisms regulating this transition of NPC division modes have not been clarified yet. To elucidate the molecular mechanisms regulating this transition, I analyzed the roles of PAR3, the evolutionarily conserved polarity protein, in mouse NPCs at E10.5. I found that PAR3-deficient NPCs were significantly biased to self-renewal divisions and altered expression of several transcription factors. These observations suggest that PAR3 is involved in the regulation of the transition of NPC division modes in mice.

Research Products

• [Journal Article] Spatial regulation of VEGF receptor endocytosis in angiogenesis (2013)
  • Author(s): Nakayama, M., Nakayama, A., van Lessen, M., Yamamoto, H., Hoffmann, S., Drexler, H.C., Itoh, N., Hirose, T., Breier, G., Vestweber, D., Cooper, J.A., Ohno, S., Kaibuchi, K. & Adams, R.H
  • Journal Title: endocytosis in angiogenesis. Nat Cell Biol Volume: 15 Pages: 249-60
  • DOI: DOI:10.1038/ncb2679
  • Peer Reviewed
• [Journal Article] Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis (2012)
  • Author(s): den, S., van Riel, W.E., Schafer, R., Song, J.Y., Hirose, T., Ohno, S. & Collard, J.G
  • Journal Title: Cancer Cell Volume: 22 Pages: 389-403
  • DOI: DOI:10.1016/j.ccr.2012.08.004
  • Peer Reviewed
• [Presentation] 神経幹前駆細胞の運命決定に対する PAR3 の機能解析 (2012)
  • Author(s): 廣瀬智威、杉谷善信、日下智保、野田哲生、大野茂男
  • Organizer: 包括型脳科学研究推進支援ネットワーク」第3回夏のワークショップ
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2012-07-26
• [Presentation] PAR3 Is Required For Restricting Symmetric Proliferative Divisions In Mouse Neural Precursor Cells (2012)
  • Author(s): Hirose T, Sugitani Y, Kusaka C, Noda T and Ohno S
  • Organizer: 新学術領域研究「神経細胞の多様性と大脳新皮質の構築」第1回国際シンポジウム
  • Place of Presentation: 岡崎カンファレンスセンター (愛知県)
  • Year and Date: 2012-03-12
• [Presentation] PAR3 is required for restricting symmetric proliferative divisions in mouse neural precursor cells (2011)
  • Author(s): 廣瀬智威、杉谷善信、日下智保、野田哲生、大野茂男
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13