2013 Fiscal Year Final Research Report
Comprehensive identification and function analysis of new autophagy-related genes using fly cultured cells
Project/Area Number |
23790371
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pathological medical chemistry
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Research Institution | Kyushu University |
Principal Investigator |
HIROTA Yuko 九州大学, 薬学研究科(研究院), 助教 (50588259)
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Project Period (FY) |
2011 – 2013
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Keywords | オートファジー / マイトファジー / リソソーム |
Research Abstract |
Autophagy is a catabolic process that degrades cytoplasmic components and organelles, which utilizes lysosomal degradative function. We observed macroautophagy and mitophagy using a sensitive method in HeLa cells using the pH-sensitive fluorescent protein Keima. Using this method, we found that mitophagy was barely induced by carbonyl cyanide m-chlorophenylhydrazine treatment, which is widely used as an inducer of Parkin-related mitophagy, whereas a small but modest level of mitochondria was degraded by mitophagy with starvation or hypoxia. Mitophagy induced by starvation or hypoxia was not suppressed by knockdown of Atg7 and Atg12, or LC3, which are essential for conventional autophagy. Finally, we found that the MAPKs ERK2 and p38 were required for mitophagy. Based on these findings, we conclude that mitophagy in mammalian cells predominantly belongs to an alternative autophagy, and requires ERK2 and p38 signaling pathways.
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Research Products
(11 results)
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[Journal Article] The Tor and Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg322014
Author(s)
Aihara, M., Jin, X., Kurihara, Y., Yoshida, Y., Matsushima, Y., Oku, M., Hirota, Y., Saigusa, T., Aoki, Y., Uchiumi, T., Yamamoto, T., Sakai, Y., Kang, D., and Kanki, T.
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Journal Title
J. Cell Sci
Volume: (In Press)
Peer Reviewed
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[Journal Article] Casein kinase 2 is essential for mitophagy2013
Author(s)
Kanki, T., Kurihara, Y., Jin, X., Goda, T., Ono, Y., Aihara, M., Hirota, Y., Saigusa, T., Aoki, Y., Uchiumi, T., and Kang, D.
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Journal Title
EMBO Rep
Volume: 14
Pages: 749-845
DOI
Peer Reviewed
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[Journal Article] Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast2012
Author(s)
Kurihara, Y., Kanki, T., Aoki, Y., Hirota Y., Saigusa, T., Uchiumi, T., and Kang, D.
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 3265-3272
DOI
Peer Reviewed
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[Journal Article] Phosphorylation of Serine 114 on Atg32 mediates mitophagy2011
Author(s)
Aoki, Y., Kanki, T., Hirota, Y., Kurihara, Y., Saigusa, T., Uchiumi, T., and Kang, D.
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Journal Title
Mol. Biol. Cell
Volume: 22
Pages: 3206-3217
DOI
Peer Reviewed
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[Presentation] Phosphorylation of Ser114 on Atg32 mediates mitophagy2011
Author(s)
Aoki, Y., Kanki, T., Hirota, Y., Kurihara, Y., Saigusa, T., and Kang, D.
Organizer
European Meeteing on Mitochondrial Pathology
Place of Presentation
Spain
Year and Date
2011-06-20
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