Regulation mechanism of mammalian sex differentiation and disorders of sex development

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790386
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human genetics
• Research Institution: Kobe University
• Principal Investigator: YOKOYAMA Toshifumi 神戸大学, (連合)農学研究科(研究院), 助教 (10380156)
• Project Period (FY): 2011 – 2013
• Keywords: 性分化 / 性分化異常 / ターナーモザイク

Research Abstract

We examined the relationships between regulation mechanisms of gonadal sex differentiation and disorders of sex development (DSD). In the DSD line mice (Ypos), inappropriate spatiotemporal expression of sex differentiation related factors in the developing gonad cause different phenotype from the same genetic background. In the Turner mosaic patient, the expression of sex differentiation related genes differed by gonads on both side, and the mRNA expression level was not consisting with mosaic ratio or protein expression. In addition, both three-dimensional structure and cell adherence were indispensable for the maintenance of the Sertoli cell characteristics under the culture condition. Furthermore, it became clear that GATA4 regulates expression of DMRT1 in the developing chicken gonads, and DMRT1 has various roles in the chicken embryo.

Research Products

• [Journal Article] Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Mullerian ducts (2014)
  • Author(s): Omotehara T, Smith CA, Mantani Y, Kobayashi Y, Tatsumi A, Nagahara D, Hashimoto R, Hirano T, Umemura Y, Yokoyama T, Kitagawa H, Hoshi N
  • Journal Title: Poult Sci Volume: 93(4) Pages: 953-958
  • DOI: 10.3382/ps.2013-03672
  • Peer Reviewed
• [Presentation] Effect of Cell-Cell contact on the maturation of Sertoli cells (2013)
  • Author(s): 横山俊史,金聖民,Ferhat Ulu,山崎由起子
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
  • Year and Date: 20130920-22
• [Presentation] 性腺分化とその破綻メカニズムに関する分子形態学的研究 (2013)
  • Author(s): 表原拓也,木下今日子,長原大知,段上めぐみ,徳本順子,小林泰丈,辰巳敬哉,橋本理恵,平野哲史,梅村ゆりあ,横山俊史,北川浩,星信彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
  • Year and Date: 20130920-22
• [Presentation] Cell-Cell contact is essential for Sertoli cell maturation (2013)
  • Author(s): Toshifumi Yokoyama, Sung-Min Kim, Ferhat Ulu, and Yukiko Yamazaki
  • Organizer: Biomedical Sciences and Health Disparities Symposium
  • Place of Presentation: University of Hawaii, USA
  • Year and Date: 20130408-09
• [Presentation] セルトリ細胞の成熟に対する細胞接着の影響 (2013)
  • Author(s): 横山俊史,金聖民,Ferhat Ulu,山崎由起子
  • Organizer: 第89回日本解剖学会近畿支部学術集会
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2013-11-30
• [Presentation] ターナーモザイク患者の性腺分化とその破綻機構の分子基盤の解明 (2012)
  • Author(s): 長原大知,森實真由美,蛯名康彦,木下今日子,段上めぐみ,徳本順子,安田玲子,表原拓也,小林泰丈,辰巳敬哉,横山俊史,北川浩,山田秀人,星信彦
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 20120914-16
• [Presentation] ニワトリ胚における性分化関連遺伝子の時空間的発現に関する分子形態学的研究 (2012)
  • Author(s): 表原拓也,木下今日子,長原大知,段上めぐみ,徳本順子,小林泰丈,辰巳敬哉,橋本理恵,平野哲史,梅村ゆりあ,横山俊史,北川浩,星信彦
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 20120914-16
• [Presentation] 性腺分化と性分化カスケードに関する分子形態学的研究 (2012)
  • Author(s): 木下今日子,長原大知,段上めぐみ,徳本順子,表原拓也,小林泰丈,辰巳敬哉,横山俊史,北川浩,星信彦
  • Organizer: 第88回日本解剖学会近畿支部学術集会
  • Place of Presentation: 神戸大学
  • Year and Date: 2012-12-01