Analysis of gastric and intestinal phenotype of gastric cancer in asymmetrical cell division

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Hiroshima University
• Principal Investigator: OUE Naohide 広島大学, 医歯薬保健学研究院, 准教授 (60346484)
• Project Period (FY): 2011 – 2012
• Keywords: 分子病理 / 腫瘍 / 胃癌 / 形質発現

Research Abstract

In the present study, we analyzed cancer stem cell marker and transition of gastric or intestinal phenotype in gastric cancer. GeneChip analysis revealed that KLF-2 and Ets-2, which are transcriptional factor, were over-expressed in side-population cells. We also found that integrin a2 isover-expressed in side-population cells. Fraction of side-population cell was reduced by knockdown of KLF-5 and Ets-2. Integrin a2 was expressed in cells showing perpendicular division. These molecules are potential marker for cancer stem cells.

Research Products

• [Journal Article] Expression of olfactomedin 4 and claudin-18 in serrated neoplasia of the colorectum: a characteristic pattern is associated with sessile serrated lesion (2013)
  • Author(s): Sentani K, Sakamoto N, Shimamoto F, Anami K, Oue N, Yasui W
  • Journal Title: Histopathology Volume: 62 Pages: 1018-27
  • Peer Reviewed
• [Journal Article] MicroRNA-155 is a predictive marker for survival in patients with clear cell renal cell carcinoma (2013)
  • Author(s): Shinmei S, Sakamoto N, Goto K, Sentani K, Anami K, Hayashi T, Teishima J, Matsubara A, Oue N, Kitadai Y, Yasui W
  • Journal Title: Int J Urol Volume: 20 Pages: 468-77
  • Peer Reviewed
• [Journal Article] The Search for Secreted Proteins in Prostate Cancer by the Escherichia coli Ampicillin Secretion Trap: Expression of NBL1 Is Highly Restricted to the Prostate and Is Related to Cancer Progression (2013)
  • Author(s): Hayashi T, Sentani K, Oue N, Ohara S, Teishima J, Anami K, Sakamoto N, Matsubara A, Yasui W
  • Journal Title: Pathobiology Volume: 80 Pages: 60-9
  • Peer Reviewed
• [Journal Article] NOS2 enhances KRAS-induced lung carcinogenesis, inflammation and microRNA-21 expression (2013)
  • Author(s): Okayama H, Saito M, Oue N, Weiss JM, Stauffer J, Takenoshita S, Wiltrout RH, Hussain SP, Harris CC
  • Journal Title: Int J Cancer Volume: 132 Pages: 9-18
  • Peer Reviewed
• [Journal Article] Reg IV Is a Direct Target of Intestinal Transcriptional Factor CDX2 in Gastric Cancer (2012)
  • Author(s): Naito Y, Oue N, Hinoi T, Sakamoto N, Sentani K, Ohdan H, Yanagihara K, Sasaki H, Yasui W
  • Journal Title: PLoS One Volume: 7 Pages: e47545
  • DOI: DOI:10.1371/journal.pone.0047545
  • Peer Reviewed
• [Journal Article] Infantile adenomyoma subclinically excreted into the patient's diaper (2012)
  • Author(s): Anami K, Sentani K, Sakamoto N, Uraoka N, Oue N, Yasui W
  • Journal Title: Pathol Int Volume: 62 Pages: 532-7
  • Peer Reviewed
• [Journal Article] Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer (2012)
  • Author(s): Sakamoto N, Oue N, Sentani K, Anami K, Uraoka N, Naito Y, Oo HZ, Hinoi T, Ohdan H, Yanagihara K, Aoyagi K, Sasaki H, Yasui W
  • Journal Title: Cancer Sci Volume: 103 Pages: 1744-50
  • Peer Reviewed
• [Journal Article] Upregulation of HOXA10 in gastric cancer with the intestinal mucin phenotype: Reduction during tumor progression and favorable prognosis (2012)
  • Author(s): Sentani K, Oue N, Naito Y, Sakamoto N, Anami K, Oo HZ, Uraoka N, Aoyagi K, Sasaki H, Yasui W
  • Journal Title: Carcinogenesis Volume: 33 Pages: 1081-8
  • Peer Reviewed
• [Journal Article] Olfactomedin-4 is a glycoprotein secreted into mucus in active IBD (2012)
  • Author(s): Gersemann M, Becker S, Nuding S, Antoni L, Ott G, Fritz P, Oue N, Yasui W, Wehkamp J, Stange EF
  • Journal Title: J Crohns Colitis Volume: 6 Pages: 425-34
  • Peer Reviewed
• [Journal Article] Cytokeratin 7 is a predictive marker for survival and effect of adjuvant chemotherapy in patients with esophageal squamous cell carcinoma (2012)
  • Author(s): Oue N, Noguchi T, Anami K, Kitano S, Sakamoto N, Sentani K, Uraoka N, Aoyagi K, Yoshida T, Sasaki H, Yasui W
  • Journal Title: Ann Surg Oncol Volume: 19 Pages: 1902-10
  • Peer Reviewed
• [Journal Article] Cytokeratin expression profiling in gastric carcinoma: clinicopathologic significance and comparison with tumor-associated molecules (2012)
  • Author(s): Takami H, Sentani K, Matsuda M, Oue N, Sakamoto N, Yasui W
  • Journal Title: Pathobiology Volume: 79 Pages: 154-161
  • Peer Reviewed
• [Journal Article] Expression of cancer stem cell markers ALDH1, CD44 and CD133 in primary tumor and lymph node metastasis of gastric cancer (2012)
  • Author(s): Wakamatsu Y, Sakamoto N, Oo HZ, Naito Y, Uraoka N, Anami K, Sentani K, Oue N, Yasui W
  • Journal Title: Pathol Int Volume: 62 Pages: 112-119
  • Peer Reviewed
• [Journal Article] Desmocollin 2 is a new immunohistochemical marker indicative of squamous differentiation in urothelial carcinoma (2011)
  • Author(s): Hayashi T, Sentani K, Oue N, Anami K, Sakamoto N, Ohara S, Teishima J, Noguchi T, Nakayama H, Taniyama K, Matsubara A, Yasui W
  • Journal Title: Histopathology Volume: 59 Pages: 710-21
  • Peer Reviewed
• [Journal Article] Molecular pathology of gastric cancer: Research and practice (2011)
  • Author(s): Yasui W, Sentani K, Sakamoto N, Anami K, Naito Y, Oue N
  • Journal Title: Pathol Res Pract Volume: 207 Pages: 608-12
  • Peer Reviewed
• [Journal Article] Identification of transmembrane protein in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of CDON is involved in tumor cell growth and invasion (2011)
  • Author(s): Hayashi T, Oue N, Sakamoto N, Anami K, Oo HZ, Sentani K, Ohara S, Teishima J, Matsubara A, Yasui W
  • Journal Title: Pathobiology Volume: 78 Pages: 277-84
  • Peer Reviewed
• [Journal Article] Serum concentration and expression of Reg IV in patients with esophageal cancer: age-related elevation of serum Reg IV concentration (2011)
  • Author(s): Oue N, Noguchi T, Anami K, Sentani K, Sakamoto N, Uraoka N, Wakamatsu Y, Sasaki H and Yasui W
  • Journal Title: Oncol Lett Volume: 2(2) Pages: 235-239
  • Peer Reviewed
• [Journal Article] Combination of protein coding and non-coding gene expression as a robust prognostic classifier in stage I lung adenocarcinoma
  • Author(s): Akagi I, Okayama H, Schetter AJ, Robles AI, Kohno T, Bowman ED, Kazandjian D, Welsh JA, Oue N, Saito M, Miyashita M, Uchida E, Takizawa T, Takenoshita S, Skaug V, Mollerup S, Haugen A, Yokota J, Harris CC
  • Journal Title: Cancer Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Decreased FANCJ caused by 5FU contributes to the increased sensitivity to oxaliplatin in gastric cancer cells
  • Author(s): Mori R, Yoshida K, Tanahashi T, Yawata K, Kato J, Okumura N, Tsutani Y, Okada M, Oue N, Yasui W
  • Journal Title: Gastric Cancer Volume: (in press)
  • Peer Reviewed
• [Presentation] 食道扁平上皮癌におけるサイトケラチン7の免疫染色は術後補助化学療法の効果予 測マーカー、予後予測マーカーとして有用である (2012)
  • Author(s): 大上直秀
  • Organizer: 第23回日本消化器癌発生学会, ワークショップ 4
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-15
• [Presentation] Alteration of microRNA expression in gastrointestinal cancer. (2012)
  • Author(s): Oue N, Sakamoto N, Naito Y, Sentani K, Harris CC and Yasui W
  • Organizer: The 22nd Hiroshima Cancer Seminar and the 4th Japanese Association for RNA interference Joint International Symposium "MicroRNAs in cancer"
  • Place of Presentation: Hiroshima (Japan)
  • Year and Date: 2012-08-30
• [Presentation] SAGE 法、CAST 法から明らかになった胃型・腸型胃癌の生物学的・臨床的特徴 (2012)
  • Author(s): 大上直秀
  • Organizer: 第5回消化器 Research Seminar
  • Place of Presentation: 広島
  • Year and Date: 2012-06-19
• [Presentation] 消化器癌発生・進展と微小環境:SEC11A の胃癌における高発現と TGF-α/EGF 分泌促進作用 (2011)
  • Author(s): 大上直秀
  • Organizer: 第22回日本消化器癌発生学会, ワークショップ 1
  • Place of Presentation: 佐賀
  • Year and Date: 20111125-26
• [Presentation] 病理セッション「胃癌における HER2 評価法と当研究室における経験」 (2011)
  • Author(s): 大上直秀
  • Organizer: 第2回 HER2 胃癌診断セミナー
  • Place of Presentation: 広島
  • Year and Date: 2011-09-08
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/byori1/
• [Patent(Industrial Property Rights)] 新規癌検出方法、癌検出器具、および癌検出キット (2012)
  • Inventor(s): 安井弥、大上直秀
  • Industrial Property Rights Holder: 国立大学法人広島大学
  • Industrial Property Number: 特許第5055543 号
  • Acquisition Date: 2012-08-10