2012 Fiscal Year Final Research Report
The significance of cell-cell interaction between macrophages and cancer cells in tumor development
| Project/Area Number |
23790407
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 分子病理 |
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| Research Abstract |
In this study , we investigated the significance of tumor-associated macrophages (TAM) inhuman malignant tumors including glioma and adult T cell lymphoma/leukemia (ATLL).As the result of immunostaining, many TAMs were detected in tumor tissues and highnumber of CD163-positive TAMs was well associated to worse clinical prognosis in gliomaand ATLL. By means of in vitro coculture study , we found glioma cells and ATLL cells weresignificantly activated by coculture with M2 macrophages. Direct cell-cell contact wasimportant in tumor cell activation. Direct contact with tumor cells induced significantmacrophage activation and which in turn soluble factors from macrophages stimulatedtumor cells. Soluble factors such as C5a、TNF-a、GRO-a,IL-6、I-309 were secreted fromactivated macrophages. In glima, membrane type M-CSF on tumor cell surface couldstimulate macrophage activation strongly . In ATLL, detail mechanisms have never beenuncovered but CD163 is considered to be involved in cell-cell interaction.
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