An investigation into functional roles of microRNA inthe animal model of malignant peritoneal mesothelioma

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790440
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Nagoya University
• Principal Investigator: OKAZAKI Yasumasa 名古屋大学, 医学系研究科, 助教 (30403489)
• Project Period (FY): 2011 – 2012
• Keywords: microRNA / 動物モデル / 悪性中皮腫 / 鉄化合物 / アスベスト / Twist-1

Research Abstract

Preliminary experiment of microRNA (miRNA) microarray disclosed miRNA-199/214 as a hallmark of iron saccharate-induced sarcomatoid malignant mesothelioma. With the aid of this grant, elevation of miRNA-199/214 was confirmed by real-time PCR and ISH. Twist-1, which transcribes miRNA-199/214, was detected in sacromatoid mesothelioma and asbestos-induced malignant peritoneal mesothelioma and cultured cell lines. Transfection of miR-199/214 into immortalized mesothelial cell line increased cell growth, providing evidence of a functional role of miRNA-199/214.

Research Products

• [Journal Article] Metamorphosis of mesothelial cells with active horizontal motility in tissue culture (2013)
  • Author(s): Nagai H, Chew SH, Okazaki Y, Funahashi S, Namba T, Kato T, Enomoto A, Jiang L, Akatsuka S, *Toyokuni S
  • Journal Title: Sci Rep. Volume: 3 Pages: 1144
  • Peer Reviewed
• [Journal Article] Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats (2012)
  • Author(s): Okazaki Y, Kono I, Kuriki T, Funahashi S, Fushimi S, Iqbal M, Okada S, *Toyokuni S
  • Journal Title: J Clin Biochem Nutr Volume: 51(2) Pages: 84-90
  • Peer Reviewed
• [Journal Article] Diameter and rigidity of multi-walled carbon nanotubes are critical factors in mesothelial injury and carcinogenesis (2011)
  • Author(s): Nagai H, Okazaki Y, Chew SH, Misawa N, Yamashita Y, Akatsuka S, Ishihara T, Yamashita K, Yoshikawa Y, Yasui H, Jiang L, Ohara H, Takahashi T, Ichihara G, Kostarelos K, Miyata Y, Shinohara H, *Toyokuni S
  • Journal Title: Proc Natl Acad Sci USA Volume: 108(49) Pages: E1330-1338
  • Peer Reviewed
• [Journal Article] Asbestos surface provides a niche for oxidative modification. (2011)
  • Author(s): Nagai H, Ishihara T, Lee WH, Ohara H, Okazaki Y, Okawa K, *Toyokuni S.
  • Journal Title: Cancer Sci Volume: 102(12) Pages: 2118-2125
  • Peer Reviewed
• [Journal Article] CD146 and IMP3 predict prognosis of asbestos-induced rat mesothelioma
  • Author(s): Okazaki Y, Nagai H, Chew SH, Jiang L, Funahashi S, Tsujimura T, *Toyokuni S
  • Journal Title: Cancer Sci Volume: (in press)
  • Peer Reviewed
• [Presentation] (2012)
  • Author(s): 岡崎泰昌
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] (2012)
  • Author(s): 岡崎泰昌
  • Organizer: 第36回鉄バイオサイエンス学会
  • Place of Presentation: 札幌
  • Year and Date: 20120901-02
• [Presentation] (2012)
  • Author(s): 岡崎泰昌
  • Organizer: 第65回酸化ストレス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 20120607-08
• [Presentation] (2012)
  • Author(s): 岡崎泰昌
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20120426-28
• [Presentation] (2012)
  • Author(s): 岡崎泰昌
  • Organizer: 第3回 JMIG (Japan Mesothelioma Interest Group) 研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-11-17
• [Presentation] (2011)
  • Author(s): 岡崎泰昌
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Presentation] (2011)
  • Author(s): 岡崎泰昌
  • Organizer: 第35回鉄バイオサイエンス学会
  • Place of Presentation: 東京
  • Year and Date: 20110910-11
• [Presentation] (2011)
  • Author(s): OKAZAKI YASUMASA
  • Organizer: 5thBiennial Meeting of SFRR (Society for Free Radical Research) Asia
  • Place of Presentation: Kagoshima
  • Year and Date: 20110831-0904
• [Presentation] (2011)
  • Author(s): 岡崎泰昌
  • Organizer: 第64回酸化ストレス学会学術集会
  • Place of Presentation: 留寿都
  • Year and Date: 20110702-03
• [Presentation] (2011)
  • Author(s): 岡崎泰昌
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110428-30
• [Presentation] (2011)
  • Author(s): 岡崎泰昌
  • Organizer: 第11回AOB (AntiOxidant Biofactor) 研究会
  • Place of Presentation: 留寿都
  • Year and Date: 2011-07-01
• [Presentation] (2011)
  • Author(s): 岡崎泰昌
  • Organizer: 第2回JMIG研究会
  • Place of Presentation: 大阪
  • Year and Date: 2011-01-28
• [Book] 第4章呼吸器系第1節誘発モデル第2項 中皮腫第6章泌尿生殖系第1節腎臓第2項誘発モデル (2012)
  • Author(s): 中村卓郎
  • Total Pages: 383-392,549-559
  • Publisher: エル・アイ・シー
• [Book] 鉄の発癌に及ぼす役割 (2012)
  • Author(s): 日本鉄バイオサイエンス学会
  • Total Pages: 70-89
  • Publisher: 医薬ジャーナル社
• [Book] 第17 章 膵臓 (2011)
  • Author(s): 豊國伸哉 高橋雅英
  • Total Pages: 779-790
  • Publisher: 丸善出版