2012 Fiscal Year Final Research Report
A Establishment of a leukemina model of cynomolgus macaque
| Project/Area Number |
23790442
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | iPS細胞 / 白血病 / 動物モデル |
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| Research Abstract |
We successfully induced CD34 positive hematopoietic stem cells (iCD34) from induced pluripotent stem cells (iPS cells) of a cynomolgus macaque which homozygously have a certain haplotype of major histocompatibility complex (Mafa-HT1). Next, we transduced well-known leukemic genes, AML1/ETO and FLT3mutant, or BCR/ABL, to the iCD34 by retrovirus. These two types of iCD34 (leukemic iCD34) cells with the leukemic genes could form many colonies in methylcellulose media and be cultured for long term without any cytokines. These leukemic iCD34 could be alive in NOD/Shi-scid, IL-2Rγnull mouse (NOG mouse) after intrabonemarrow-bonemarrow transplantation (IBM-BMT). We are preparing experiments in which a bonemarrow transplantation of the leukemic iCD34 with AML1/ETO+FLT3mutant to a cynomolgusmacaque with heterogygous MHC.
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[Journal Article] Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A*052:022012
Author(s)
Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K.
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Journal Title
PLos ONE
Volume: 7
Pages: e37220
Peer Reviewed
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