2013 Fiscal Year Final Research Report
Development of a novel orthotopic transplantation model of human small cell lung cancer metastasis.
| Project/Area Number |
23790452
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Microbial Chemistry Research Foundation |
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Principal Investigator |
SAKAMOTO Shuichi 公益財団法人微生物化学研究会, 微生物化学研究所 沼津支所, 主任研究員 (60346070)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 癌転移 / 小細胞肺癌 / 同所移植 / HGF |
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| Research Abstract |
Because small cell lung cancer (SCLC) is an aggressive cancer with highly metastatic activity, it is important to reveal the molecular basis of the SCLC metastasis for improvement of SCLC therapeutics. In this study, we developed a new in vivo orthotopic model for SCLC metastasis and investigated the molecular mechanism of metastasis using the model. We found that cells used in our model expressed both HGF and its receptor c-MET, and that condition medium of the cells enhanced motility of them. These data suggest the existence of an HGF/MET autocrine loop in our model as in SCLC patients.
Moreover, we established a new subclone of the cells with higher metastatitic activity through in vivo selection cycles, and successfully improved our model using this subclone.
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Research Products
(7 results)
