2013 Fiscal Year Final Research Report
Elucidation of the carbohydrate recognization mechanisms by intestinal protozoa using MUC2 glycopeptides
| Project/Area Number |
23790460
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
KATO Kentaro 長崎大学, 熱帯医学研究所, 助教 (50508885)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 原虫 / 腸管粘膜糖鎖 |
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| Research Abstract |
Among intestinal protozoan parasites, Entamoeba histolytica infects large intestine and Cryptosporidium parvum infects small intestine via carbohydrates of the mucus. To elucidate the reason for this difference in the site of infections, recombinant lectins expressed in E. coli and 56 kinds of synthetic glycopeptides were prepared.
However, it was difficult to obtain an active Entamoeba lectin. Therefore, a new assay using Entamoeba histolytica membrane and neo-glycoproteins was established. The reason for mouse strain-dependent susceptibility to Entamoeba histolytica infection was elucidated by using this assay.
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