Genome analysis of bacteriophage toward botulinum neurotoxin-transfer mechanism

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790479
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: SAKAGUCHI Yoshihiko 宮崎大学, IR 推進機構, 助教 (70403491)
• Project Period (FY): 2011 – 2012
• Keywords: ボツリヌス菌 / ボツリヌス毒素遺伝子 / バクテリオファージ / プラスミド / ファージ変換 / 接合伝達 / チューブリン / 分配

Research Abstract

Phage c-st is typical Clostridium botulinumtype C neurotoxin-converting phage. Although the phage c-st genome has been sequenced, no much information is available in its biology and genome. To extend our understanding of horizontal gene transfer by this phage, we investigated in the core genome of phage c-st. First, analyzing the phage structural proteins, the genome module for the structural proteins was 98.5-157.5 kbp of phage c-st genome. Second, comparing the genome of phage c-st with that of a related phage c-468, the 43-57 kbp and 116.5-123 kbp of phage c-st genome were very similar to phage 468 genome. Third, the essential genes for phage genome partitioning were revealed to be located 175.5-178.5 kbp of phage c-st genome. According to these results, I speculated that the core genome was the 1-43 kbp, 57-116.5 kbp, and 123-186 kbp regions of phage c-st genome.

Research Products

• [Journal Article] Phospholipase C produced by Clostridium botulinumtypes C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin (2013)
  • Author(s): 2Fatmawati, N. N., Sakaguchi, Y., Suzuki, T., Oda, M., Shimizu, K., Yamamoto, Y., Sakurai, J., Matsushita, O., Oguma, K.
  • Journal Title: Acta Med. Okayama Volume: 67(1) Pages: 9-18
  • URL: http://www.lib.okayama-u.ac.jp/www/acta/pdf/67_1_9.pdf
  • Peer Reviewed
• [Journal Article] 1Oliva, M. A., Martin-Galiano, A. J., Sakaguchi, Y., Andreu, J. M (2012)
  • Author(s): 1Oliva, M. A., Martin-Galiano, A. J., Sakaguchi, Y., Andreu, J. M
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 109(20) Pages: 7711-7716
  • URL: http ://www.pnas.org/cgi/doi/10.1073/pnas.1121546109.
  • Peer Reviewed
• [Journal Article] TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding (2011)
  • Author(s): Sakaguchi, M., Murata, H., Yamamoto, K., Ono, T., Sakaguchi, Y., Motoyama, A., Hibino, T., Kataoka, K., Huh, N. H.
  • Journal Title: PLoS One Volume: 6(8) Pages: e23132
  • DOI: DOI:10.1371/journal.pone.0023132
  • Peer Reviewed
• [Presentation] The search for core-genome of Clostridium botulinumbacteriophage converting type C neurotoxin (2013)
  • Author(s): Sakaguchi, Y., Uchiyama, J., Ogura, Y., Yamamoto, Y., Suzuki, T., Orita, K., Tsuda, C., Matsuzaki, S., Hayashi, T., Oguma, K
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
  • Year and Date: 20130318-20
• [Presentation] The complete sequence of plasmid encoding neurotoxin gene in Clostridium botulinumtype B strain (2013)
  • Author(s): Hosomi, K., Sakaguchi, Y., Goto, K., Nakamura, K., Kohda, T., Mukamoto, M., Iida, T., Ogura, Y., Hayashi, T., Kozaki, S.
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
  • Year and Date: 20130318-20
• [Presentation] Morphological and genomic analysis of Helicobacter pyloribacteriophage KHP30 (2013)
  • Author(s): Uchiyama, J., Takeuchi, H., Sakaguchi, Y., Ujihara, T., Uchiyama, I., Daibata, M., Matsuzaki, S
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
  • Year and Date: 20130318-20
• [Presentation] Analysis of pseudolysogenic lifecycle of Clostridium botulinumphage c-st, based on comparative genomics of wild-type and long-term-incubated mutants phages (2012)
  • Author(s): 4Sakaguchi, Y., Oliva, M. A., Martin-Galiano, A. J., Andreu, J. M., Sakaguchi, M., Uchiyama, J., Ogura, Y., Yamamoto, Y., Suzuki, T., Orita, K., Tsuda, C., Matsuzaki, S., Hayashi, T., Oguma, K.
  • Organizer: The 35th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka
  • Year and Date: 20121211-14
• [Presentation] C型ボツリヌス毒素変換ファージの構造タンパク質の解析 (2012)
  • Author(s): 阪口義彦、内山淳平、小椋義俊、山本由弥子、鈴木智典、阪口政清、織田華絵、津田千秋、松崎茂展、林 哲也、小熊惠二
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
  • Year and Date: 20120919-20
• [Presentation] Improved adsorption of Enterococcus faecalisbacteriophage ΦEF24C caused by a point mutation in a tail fiber gene (2012)
  • Author(s): Uchiyama, J., Sakaguchi, Y., Takemura-Uchiyama, I., Kato, S., Satoh, M., Ujihara, T., Daibata, M., Matsuzaki, S
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
  • Year and Date: 20120919-20
• [Presentation] ピロリ菌バクテリオファージの分離・解析 (2012)
  • Author(s): 内山淳平、竹内 啓晃、加藤伸一郎、阪口義彦、蒲生啓司、氏原隆子、内山伊代、大畑雅典、松崎茂展
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
  • Year and Date: 20120919-20
• [Book] 医薬ジャーナル社「病原菌の今日的意味 改訂 4 版 (2011)
  • Author(s): 小熊惠二、阪口義彦、西河 淳
  • Total Pages: 259-288
  • Publisher: ボツリヌス菌 (Clostridium botulinum)
• [Remarks] 宮崎大学型若手研究リーダー育成モデル
  • URL: http://www.miyazaki-u.ac.jp/ir/?p=92