Comprehensive identification of host factors that involve in Ebolavirus entry

2014 Fiscal Year Final Research Report

• Project/Area Number: 23790493
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Hokkaido University
• Principal Investigator: NANBO Asuka 北海道大学, 医学(系)研究科(研究院), 准教授 (60359487)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: エボラウイルス侵入機構の分子基盤

Outline of Final Research Achievements

The molecular mechanism of Ebolavirus (EBOV) entry remains to be elucidated. The long-term goal of our study is the comprehensive identification of host factors that involve in EBOV entry by siRNA screening. By use of fluorescently-labeled EBOV viral-like particles (VLPs), we succeeded to quantify the efficiency of adsorption of VLPs onto cell membrane, internalization of VLPs, and membrane fusion with endosomes. Currently we are performing siRNA screening with high-content imaging system. Our study may provide a new notion to understand molecular mechanism of EBOV entry and also contribute to the development of therapeutics against EBOV infection.

Free Research Field

ウイルス学