2012 Fiscal Year Final Research Report
Identification of novel mechanisms of Morbillivirus to interact withhost cells
| Project/Area Number |
23790496
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
HONDA Tomoyuki 京都大学, ウイルス研究所, 助教 (80402676)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 細胞 / 核内封入体 / 持続感染 / リン酸化 |
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| Research Abstract |
The nucleoprotein (N) is the major component of the Morvillivirusribonucleoprotein complex (RNP) and encapsidates their non-segmented negative-stranded viral genome RNA (vRNA). The viral RNA dependent RNA polymerase complex transcribes vRNA. Morbillivirus, such as measles virus (MV), establishes a persistent infection easily and forms intranuclear inclusions. N is also the major component of this viral intranuclear inclusion. We found that fusion activity of MV was reduced during the establishment of MV persistence. Then, we demonstrated that the subcellular localization of N was regulated by its phosphorylation. Finally, we established transgenic mice expressing N by a CAG promoter (N-Tg). N-Tg formed inclusion bodies in cardiac muscle and skeletal muscle. In these muscles, N-Tg showed muscle atrophy.
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